Lab Culturing Flashcards

(5 cards)

1
Q

What growth media is used

A
  • Broth
  • Agar
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2
Q

What is used to sterilise growth media

A

An autoclave- at 121C for 15 minutes

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3
Q

What are aseptic techniques

A
  • Wash hands, disinfect surfaces
  • Work near a bunsen burner to prevent airborne contamination
  • Flame neck of bottle
  • Minimise lid opening
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4
Q

What is inoculation

A

The process of introducing microorganisms into a suitable growth medium

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5
Q

How can cultures be inoculated

A
  • Streaking: drag loop across agar
  • Seeding: Pipette drop onto agar
  • Spreading: Use glass spreader
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