Lab - Differentials, Estimates & Morphologies

Question 1

Assessment of the fields is done in a systematic manner using a “_____” search pattern

Answer 1

Battlement

Question 2

Where do you start the differential on the smear slide?

Answer 2

As deep into the feathered edge as possible, while still keeping in the monolayer

Question 3

What sample do you use for a blood smear

Answer 3

EDTA (whole blood)

Question 4

During a differential, what do you do under the 10x objective?

Answer 4

Look at smear quality, staining quality, cellular distribution, abnormal WBC populations, platelet clumping and fibrin

Question 5

T/F you only have to scan the monolayer under the 10x objective

Answer 5

False - also scan the edges and the body, feathered edge and body of smear

Question 6

WBC estimates are completed under what objective

Answer 6

40x

Question 7

How do you perform a WBC estimate?

Answer 7

Assess 10 fields and count how many WBCs present. Divide this number by 10 and multiply by 2

Question 8

If you count 60 WBCs in 10 fields, what is the WBC estimate?

Answer 8

(60 / 10) *2 = 12.0 * 10^9 / L

Question 9

T/F

Platelet estimates are performed under 40x magnification

Answer 9

False - 100x

Question 10

T/F

Platelet estimates are completed before WBC differential & morphology and RBC morphology

Answer 10

True

Question 11

WBC differential and morphology are completed under what objective?

Answer 11

100x

Question 12

RBC morphology is completed under what objective?

Answer 12

100x

Question 13

Describe how to perform a platelet estimate

Answer 13

Assess 10 fields, the total number is counted and then divided by 10, and multiplied by 20

Question 14

If you assess 10 fields and count 40 platelets (yes this is a rlly small number), what is your platelet estimate?

Answer 14

(40/10) * 20 = 80* 10^9 / L

Question 15

How many WBCs do you count in a WBC differential

Answer 15

100

Question 16

Describe neutrophil morphology (nucleus, cytoplasm, granules if present)

Answer 16

Dark blue to purple nucleus with 3 or more lobes connected via a thin band of chromatin

Pale pink to almost colorless cytoplasm

Red to lavender small granules

Question 17

Describe
Basophil morphology (nucleus, cytoplasm, granules if present)

Answer 17

Dark blue to purple nucleus (often bilobed)

Pale blue cytoplasm

Deep purple and violet-black granules

Large granules

Question 18

Describe esosinophil
morphology (nucleus, cytoplasm, granules if present)

Answer 18

Multilobed, blue to blue-purple nucleus

Pale pink cytoplasm

Bright red/orange granules

Large granules

Question 19

Describe lymphocyte morphology (nucleus, cytoplasm, granules if present)

Answer 19

Dark, dense violet nucleus

Nucleus takes up most of cell space

Medium blue cytoplasm

Question 20

Describe monocyte
morphology (nucleus, cytoplasm, granules if present)

Answer 20

Lobated, medium purple nucleus

Chromatin not as dense

Light blue cytoplasm

Vacuoles sometimes present in cytoplasm

Question 21

Describe Erythrocyte morphology
(nucleus, cytoplasm, granules if present)

Answer 21

pink to red-orange biconcave disc

Question 22

Describe platelet
morphology (nucleus, cytoplasm, granules if present)

Answer 22

Clearly defined blue violet-purple granules in light blue cytoplasm

Question 23

Reference range: WBC count

Answer 23

4.0-11.0 * 10^9 /L

Question 24

Reference range (relative): Neutrophils

Answer 24

0.40 - 0.75

Question 25

Reference range (relative): Lymphocytes

Answer 25

0.20 - 0.45

Question 26

Reference range (relative): Monocytes

Answer 26

0.02 - 0.08

Question 27

Reference range (relative): Eosinophils

Answer 27

0.01 - 0.06

Question 28

Reference range (relative): Basophils

Answer 28

0.00 - 0.01

Question 29

Reference range (relative): Band cells

Answer 29

0.00 - 0.06

Question 30

Reference range (absolute): Neutrophils

Answer 30

2.5 - 7.5 *10^9 / L

Question 31

Reference range (absolute): Lymphocytes

Answer 31

1.5 - 3.5 *10^9 / L

Question 32

Reference range (absolute): Monocytes

Answer 32

0.0 - 0.8 *10^9 / L

Question 33

Reference range (absolute): Eosinophils

Answer 33

0.04 - 0.44 *10^9 / L

Question 34

Reference Range (absolute): Basophils

Answer 34

0.00 - 0.10 *10^9 / L

Question 35

Reference Range (absolute): Band cells

Answer 35

0.00 - 0.44 *10^9 / L

Question 36

If you count 68 neutrophils in your WBC diff, what is your relative amount?

Answer 36

0.68

Question 37

How do you calculate absolute WBC count?

Answer 37

Relative WBC count (in dec. form) * patient total WBC count (given via CBC)

Question 38

What are examples of abnormal findings of WBC morphology

Answer 38

Immature cells, abnormal nuclear shapes, inclusion bodies, damaged cells

Question 39

What are the three main RBC indices

Answer 39

Mean cell volume (MCV) Mean cell hemoglobin (MCH) Mean cell hemoglobin concentration (MCHC)

Question 40

What is the mean cell volume (MCV) calculation

Answer 40

(Hct / RBC count) *1000

Question 41

What is the mean cell hemoglobin (MCH) calculation

Answer 41

Hgb / RBC count

Question 42

What is the mean cell hemoglobin concentration (MCHC)

Answer 42

Hgb / Hct

Question 43

Reference range: MCV

Answer 43

80 - 96 fL

Question 44

Reference range: MCH

Answer 44

28 - 32 pg

Question 45

Reference range: MCHC

Answer 45

320 - 360 g/L