Lab Safety and Microscopes

Question 1

What are the potential hazard(s) to people when working with blood/urine/feces in veterinary clinics?

Answer 1

Bacteria
Viruses
Parasites

Question 2

How long should you flush your eyes if chemicals or biohazards are splashed into them?

Answer 2

At least 15 minutes

Question 3

Zoonoses

Answer 3

A disease that can be transmitted from animals to humans.

Question 4

What magnification level should you start when using a microscope?

Answer 4

The lowest

Question 5

What is included in PPE?

Answer 5

Gloves
Gown (lab coat)
Masks
Eye gear

Question 6

Infectious disease

Answer 6

Those that can be transmitted to a person, due to bacteria, viruses, parasites or other agents, which may invade a person’s body, possibly resulting in an infection

Question 7

OSHA meaning

Answer 7

-Occupational Safety and Health Administration
-Help employers and employees reduce on the job injuries and deaths

Question 8

What is included when labeling a sample?

Answer 8

Time, date, patient name, & your initials

Question 9

Magnification

Answer 9

The ratio of the size of a feature in an image (D) to the size of the feature in the actual object (d)

M= D/d

Question 10

Modern Microscopes “compound microscopes”

Answer 10

They magnify both in the objective and the ocular.
Magnification = Ocular x Objective
400x = 10 x 40

Question 11

Microscope use

Answer 11

1. Begin with the condenser up & the lowest magnification.
2. Adjust focus with the course knob, centering the object of interest.
3. Increase magnification as needed, using only fine focus at higher magnification.
4. Oil is used for the 1000x magnification, be careful to keep oil off all other lenses.
5. As magnification is increased, the light will need to be increased.
6. Adjust contrast with the iris diaphragm.