Things used in Differential stain in order
Crystal violet, Iodine, Acetone alcohol, Sulfranin
Acid-fast things used in order
use a heat-fixed smear, apply primary stain carbol fuchsin, Acid alcohol, apply counterstain (brilliant green)
Mycobacterium smegmatis color is _______
orange/yellow
pseudomonas aeruginosa color is _______
Green
Proteus mirabilis
Amber
Staphylococcus aureus color is
more yellow = more pathogenic
Escherichia coli
Off white
Streptococcus pyogenes
More white
Bacillus subtillis
Beige