Polymerase chain reaction
Produces multiple Dna from a specific sequence
Polymerase Chain reaction uses
Genetic testing
Parental testing
Thermocycler
Raises and Lowers the temp
Denature
Seperates the strands 94-96 degrees
Primer
Attaches to target dna site and hybridizes and amplifys the sequence. By providing 3’oh that dna polymerase needs for sysnthsis 50-65
Extension
Taq Polymerase binds and extends as complemtry DNA strand from each primer 72
Chelex
Binds metals in cheek sample which would denature the enzymes