Lec 4 Flashcards

Pain Measurement in Humans and Animals (64 cards)

1
Q

Objective vs Subjective

A

-objective measures give us precise responses, subjective would be too broad
-an advantage of an objective measures: not as prone to bias from sufferer or interpreter,
-an advantage of a subjective measure: usually easier to gather, we are more interested in subjective concept

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2
Q

Thermode Test

A

-deliver pain to a certain radius of skin that its applied to to
-can be heat or cold, usually heat
-can start at a level, and can ramp up
-can ask person for their threshold, or their tolerance

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3
Q

Cold Pressor Test

A

-have person put their hand in for how long they can stand it
-part of the pain comes from diminished blood flow from ur hand becoming so cold it becomes ischemic

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4
Q

Pressure Algometry

A

-device where you press on a part of someones body, and you press harder and harder
-device will tell what the highest pressure it got to

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5
Q

Threshold

A

-when whatever the experience is, turns into pain
-threshold will come before tolerance

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6
Q

Tolerance

A

-person tells the participant when to stop
-must be done for ethical reasons

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7
Q

Latency

A

-Latency for them to say their threshold and tolerance
-the latency tells us (what we are measuring) is degrees (temp)
-at what temperature is ones threshold and tolerance

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8
Q

Threshold Problem

A

its hard to decide at what precise moment a sensation develops into pain, often a guess, pain is a continuum

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9
Q

Tolerance Problem

A

its entirely context dependent, external pressure, interest, initiative all bias the results

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10
Q

Problem w/ Tolerance and Threshold

A

does not account form the emotional aspect of pain, detection bias
-human pain is neither threshold or tolerance, its ongoing, its over threshold and under tolerance, tolerance max would be death so its basically irrelevant
-the biology of threshold and tolerance has very little to do with how/what ur pain is
-only appears to be objective

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11
Q

Numeric Pain Intensity Scale

A

NRS has a 0 bc its representative of no pain, can be an integer

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12
Q

Visual Analogue Scale

A

-VAS has no numbers and words only on the pegs, person marks with a pencil where they think their pain is, the stroke is measured and then compare it to NRS pegs

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13
Q

VAS vs NRS

A

-better than NRS bc its a bit more continuous, not as suggestive, VAS prevents ppl from memorizing what they told you last time so physician can see the actual progress of pain and if treatment is working or not

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14
Q

RIght vs Left Pegs

A

-the left peg is consistent as no pain
-what the right peg is can be many different things
-for the right peg, its not clear that anyone knows how to compare anything experienced to “worst pain imaginable”

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15
Q

FACES Scale

A

sed for young children, could be argued that it doesnt go high enough, theres a smile for no hurt it should be neutral, children may confuse emotion they are feeling for how much hut they have

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16
Q

Scales Inter-rater Reliability

A

-scales work very well on the same person, people are very reliable within person at being able to tell you what they want to tell you witht heir scales
-multiple scales can be used to separate components of pain, ie. unpleasantness vs intensity
-bc these things can be dissociated from one another

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17
Q

Problems with Ratings

A

-many times when ppl are giving you a rating its not the actual rating its someone trying to get you to take them seriously (high rating) or impress the physician (low rating)
-its argued that there are confounds in rating system made by how wide the range of human experience is
-ex worst pain ive ever had is a wider range for women than men (bc of childbirth), so if womens scales are bigger, it can lead to confounds
-ppl imagine pain to be worse than it actually is with the exception of childbirth

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18
Q

Issues w/Price Pain Ranking

A

-problem with Price’s experience is that thermode peak pain ratings mean nothing compared to timelines of actual pain
-some of these pain states last for different amount of time, the suffering of a level 4 for years is worse than the suffering of level 9 for a second, thats the apples to apples problem
- ppl have only experienced one or two of these things so they cant accurately compare

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19
Q

Components of a Pain Ranking

A
  1. Ongoing Pain
  2. Allodynia
  3. Hyperalgesia
  4. Functioning
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20
Q

FLACC Scale

A

-for babies
-face, legs,activity, cry, consolability

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21
Q

Facial Pain Scale

A

-when we are in pain we grimace, grimacing is almost completely invariant, hard to alter or fake
-looking for movements in parts of the face

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22
Q

Ekman

A

you can code the facial expression of every human emotion by looking at muscle pulling and movement, made FACS (facial action coding system)

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23
Q

McGill Pain Questionnaire Development

A

-divided into categories: temporal, spatial,, punctate pressure, incisive pressure, contrictive pressure, pulling pressure, bright pain, dull pain, emotional aspects of it
-had a panel of doctors and a panel of patients order the words of each category in order of least to most intense and both panels agreed

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24
Q

McGill Pain Questionnaire

A

-the patient is asked to pick the word from each category that best describes their pain, additionally asked to rate the pain on a NRS (PPI-present pain index)
-also asked about timing of pain
-asked to point out where pain is on the body map
-the pattern fo adjectives can label different types of pain disorders to diagnose
-this never really worked

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25
Cons of MPQ
-main difference noticed is that neuropathic pain gives different descriptives than non-neuropathic pain -the PPI is the most common part of this that continues to be used -there is also a short form version of this (also includes PPI) -works better and doctors dont have a a lot of time
26
Dn4
-Neuropathic Pain Questionnaire -developped by the french -4 questions -get a point for each yes -if score is 6+ probably neuropathic, 3-6 might be neuropathic, 3> not neuropathic -why does it matter if its neuropathic or not? -bc neuropathic treatment doesnt respond to antiinflammatory meds (NSAID)
27
Oswestry Diablity Index
-pick one from each section -section one basically a verbal rating scale
28
Pain Catastrophizing Scale
-not a rating scale -the best predictor of pain and its prognosis -catastrophizing= rumination + magnification + helplessness -developed by mcsullivan -list of 13 questions, rated on a degree sliding scale, all in relation to pain -you can get a subscore for each of the categories, and a catastrophizing score
29
WOMAC
-specifically for arthiritis pain -asks about pain in different conxtexts -ask about independent problems like stiffness, disability etc
30
Quantitative Sensory Testing
-can conduct a bedside test or a primary care physician can also conduct this -if conducting this testing in the lab -specialized devices are use to test, measuring thresholds and tolerance for touch pressure, and temperature -use psychophysical methods -testing threshold and tolerance
31
Temperature Thresholds
-ppl will start to call something cool if it gets to 31, warm at 38, painfully cold at 14, and painfully hot at 43.5 (avg hottub temp 37C) -some variation person to person but not much
32
German QST
-can have an abnormal gain of function of abnormal loss of function -PHN 1 is a bunch of gains of function bc of peripheral and central nervous system -spontanous pain is not here, its symptoms accompanying spontaneous pain PHN: a bunch of loss of function -not differentiated by spontaneous pain, everyone experiences spontaneous pain -this tells us more about neuropathy than pain
33
Pain Testing in Muscles
-could test by pumping chemicals intot he muscle, or inserting electrodes intot the muscles and give ratings
34
Problems w/QST
-QST is still too subjective bc the person still tells you when they feel pain -also ppl will lie, either too look good, not disappoint, receive treatment or get drugs
35
Biomarkers
-biomarkers are accessible objective measures of pain, to use in addition to or instead of the subjective pain rating
36
Biomarkers of Pain
1. Tissue Damage 2.Cardiovascular 3. Stress-Related 4.Neural 5. Chemical 6.Molecular -BUT, none of these are true biomarkers bc they are not always concurrent with pain, may be better explained otherwise etc
37
Cardiovascular
* heart rate * blood pressure * heart rate variability
38
Stress-Related
* cortisol (etc.) * galvanic skin response
39
Neural
* EEG * microneurography * imaging (functional, structural, chemical)
40
Chemical
* substance P * b-endorphin * cystatin C * C reactive protein * nerve growth factor
41
Molecular
* DNA variants * mRNA levels
42
Problem w/DNA Variance
-bc you inherit it, they determine susceptibility to pain but have nothing to do with wether you are or arent in pain -pain is a classic gene x environment interaction -mRNA levels can go up and down, so theoretically we can, but practically, which cells mRNA would we even test? Bc each cell has different amounts, which cells would be the best determiners of that
43
fMRI as a Biomarker
-higher raters had more activity in the pain matrix (and vice versa for low rating), so brain agreed with rating -Derbyshire, fMRI on ppl who were in put in pain, ppl with hypnotically induced pain and ppl with imagined pain -found that there was a lot of overlap in the three situations, indicating that an fMRI can be fooled, you can imagine the pain which tricks the fMRI
44
Pros for Animal Testing
can conduct causation experiments * can stimulate/lesion any tissue * can assay, record from, or extract any tissue * can give unapproved drugs * can alter gene expression (temporarily or permanently) can turn particular types of neurons in particular locations on or off at will * can control environmental pre-exposures * cheaper, faster, less highly regulated * no malingering, no stoicism/machismo, no demand characteristics -give objective response -we can do things in animals that we cant do ethically to humans -all human experiments get correlational studies bc we cant control what happens to humans beforehand, with animals we can control them from conception
45
What Animals we Use
-we used dogs, cats, rabbits, mouse and rats in the 70s -now we almost exclusively use rats and mice -we got rid of using dogs and cats bc of animal cruelty laws and public outrage -we got rid of rabbits bc they are bigger so they require more resources and more money -the rat used to dominate, now the mouse has caught up, this is bc someone created a knockout gene in (one strain) of mice
46
Challenges to the Use of Animals
* they’re the “wrong” species * they don’t talk * they’re prey (wouldnt want to advertise pain and weakness) * they’re a lot tougher than we are * ethical issues
47
Deontological Ethics
The normative ethical position that judges the morality of an action based on the action’s adherence to a rule or rules
48
Consequentialist Ethics
The normative ethical position holding that the consequences of one’s conduct are the ultimate basis for any judgment about the rightness of that conduct.
49
Hot Plate Test
-a metal surface heated to above the animals threshold -the heat transfers from plate to paw, paw heats, mesure time it takes for animal to react meaning there is enough heat transferred that the paw becomes painfully hot -animals will either pick up their paw in shake it/put in mouth or jump off the plate
50
Tail Flick Test
-shine a heat lamp on tail or put tail in hot water, when the tail reached heat pain threshold it will flick its tail out -the difference is that the tail flick a spinal reflex, in the hot plate test the rat thinks about what it wants
51
Hargreaves Test
-put an animal on a glass surface and aim a heat lamp on one paw, then can compare ipsilateral and contralateral pain response and sensitivity -ppl dk if this is spinal or supraspinal
52
von Frey Filaments
-they are aseries of filaments made out of nylon that are calibrated from thin to thick such that it you increase the pressure exerted on them until the bend, at the moment of bending they exert a certain amount of force -time when they hop away from fibre (positive) or dont respond -eventually will find the fibre that cause hopping 50% of the time and that is the mechanical threshold
53
Randall-Selitto Test
-can only be done on rats bc you cant hold mice still enough to test -pressure applied to rats paw until reaction, that is threshold
54
Indirect Mechnical Assays
-putting rat on 2 forceplates and the computer tells you weight difference in each leg, seeing when rat is shifting weight to good leg -if inflict injury to forepaw, if they are holding onto a grid and you try to pull them away, infer mechanical alloydyna with how long they can hold on for -measure how much force they are putting on hind paws vs forepaws
55
Writhing Test
-inject acetic acid into the belly -count how many constrictions or how long they cosntrict for -can be reversed with Asprirn
56
Formalin Test
-formalin is very dilute formealdyhyde that causes licking behaviour -the more it licks the hind paw, the more pain inferred -there was a dip in pain before coming back full swing (early phase/interphase/late phase) -the late phase is longer lasting, and longer lasting pain has more to do with clinical pain -biphasic response of nociceptors and second order dorsal horn
57
Common Inflammagens
-carrageenan -complete Freunds adjuvant -Zymosan -mustard oil -when these are injected they have changes in sensititvyt to temp, pressure that lasts for days to weeks -they develop allodynia
58
Surgical
-partial injuries of perpherial nerves that causes neuropathic pain, surgically induced in animal -parital injury to foot nerves by cutting ⅔ nerves from the sciatic nerve, or take a chunk out of sciatic nerve, or constrict 2 spinal nerves and leave the third
59
Axotomy
-everything from the foot is cut, sciatic and saphenous nerve are cut -will produce autotomy wherein rodent will bite off its toes, measured by how many toes they bit off -this is a model of phantom limb pain -can argue that bc you severed the nerves, theres no way for affrent info to get to spinal cord so cant feel anything, including pain, but then why do they bite their toes off -a) bc they are in pain b) they dont think their foot is part of them anymore bc they can feel it
60
Chronic Neuropathic Assays
-there are many experimental surgeries that replicate neuropathic pain -can cause nerve damage by: inflaming a nerve, cause them to get demylinated, give diabetes, viruses, certain drugs (esp cancer drugs)
61
Are Animal Models of Pain Good Enough?
-exp: giving female mice repeated yeast infections and they developed vulvadynia -they dont do this anymore bc it takes too long and too labour intensive
62
3 Critisims of the status quo
1. Reflexive vs conditioned measures 2. Dont study pain related comorbidities 3. Symptoms epidemiology vs Dependent Measure Use
63
Symptoms epidemiology vs Dependent Measure Use
-we are looking into the wrong symptoms -mouse grimace scale is a measure of spontaneous pain in mice
64
Reflexive vs conditioned measures
-ex. Operant Conditioning: Motivational conflict between pain and drinking -a rat can only get dugar water by pressing its shaven cheek against a hot coil -would they rather not be in pain or have the sugar water? -ex. Classical conditioning: -alternate sides of boxes with each day, one day on floor with pain, one day on the other side with an analgesic -on test day, lift the partition between box sides, dont admit analgesic, and see where the rat sees, rat will likely stay on analgesic side