Lecture 10

Question 1

How to make a genomic library IN ORDER

Answer 1

1) DNA purification
2) Restriction enzyme
3) Plasmid
4) Transformation
5) Selection

Question 2

How to make a cDNA library IN ORDER

Answer 2

1) mRNA purification
2) poly(T)primer
3) RT-PCR
4) Restriction enzyme
5) Clone into plasmid
6) Transformation