Lecture 2: Microscopy

Question 1

Refractive power in diopters

Answer 1

1 meter/focal length of lens

Question 2

Resolution

Answer 2

d = 0.61λ/nsinα

d = resolution, smaller d = greater resolution

lamda = wavelength of light

n = refractive index of medium surrounding object

sin alpha = angle of cone of light entering objective lens

nsinalpha = numerical aperture

Question 3

Real image

Answer 3

object is placed outside the focal point

inverted, can be projected, differs in size from object

Question 4

virtual image

Answer 4

object is placed inside the focal point

not inerverted, can not be projected, can be magnified

ex: mirror image