Lecture 22: DNA Replication

Question 1

What are DNA polymerase requirements?

Answer 1

Requires:

• all four deoxyribonucleoside triphosphates (dATP, dGTP, dCTP, dTTP)
• magnesium ions Mg 2+
• a primer (sequence of bases used to initiate synthesis synthesised by primase). Growing chain only occurs by attaching deoxyribonucleoside mono phosphate to the 3’ OH end of DNA
• a template strand

Question 2

DNA polymerase 3 is essential for prokaryotic DNA replication

Answer 2

-only DNA polymerase 3 works fast enough to account for the rate of DNA replication in cells

Question 3

DNA replication by DNA polymerase

Answer 3

DNA polymerase synthesize exclusively in the 5’ to 3’ direction
-sands are anti parallel

Question 4

Much much more in this lecture that is boring as bat shit so need to go over it

Answer 4

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