Lecture 26

Question 1

Describe the lifecycle states of Caulobacter crescentus

Answer 1

1. Swarmer cells - swims in aquatic environment and find a surface to attach to. Undergoes development where it produces a ‘hold fast’ (sticks itself to a surface). At this point it CANNOT DIVIDE
2. Stalked cell - cell post attachment, only time it can divide

Question 2

Describe Illumina Sequencing in terms of Transposon location

Answer 2

It gives the transposon and a small piece of surrounding DNA allowing them to know where the Tn jumped in

Question 3

What is the effect of a Tn jumping into an essential gene?

Answer 3

No growth

Question 4

What is the effect of a Tn jumping into a high fitness ORF?

Answer 4

These aren’t essential but are good for bacterial growth, a Tn insertion will cause bacteria to grow slower

Question 5

Where might essential genes be able to tolerate inesertions and why is this?

Answer 5

• At the far end, majority of the protein has been formed and is functional at this point
• Might tolerate right at the beginning