Lecture 5

Question 1

What do metagenomic studies not require

Answer 1

No need for PCR amplification of a target gene, illumina libraries are generated directly from the DNA extracted from the sample

Question 2

What are two methods of analysis

Answer 2

Taxonomic profiling or de novo metagenome assembly

Question 3

What is metagenomic analysis sensitive to

Answer 3

Poor quality data

Question 4

How do we carry out metagenomic analysis

Answer 4

1. start off with sequence reads
2. Go down route of taxonomic profiling, taking each individual read and working out which genus is most likely to be associated with
3. or alternatively, take reads and try and piece then together
4. Assemble them in the same way as a single genome

Question 5

What does the QC stage involve

Answer 5

Removing regions of poor quality data from the reads, sequence the data