Lecture 5

Question 1

What is the effect of the anneal ing temp on PCR products?

Answer 1

as temp increases- the primers will bind less effectively=less product made

as temp decreases- the primers may bind alternative sites- non specific products

Question 2

Ligation

Answer 2

covalent join vector and insert DNA

Question 3

Transform

Answer 3

introduce ligation mixture into host cells for replication of DNA. E.coli is the hose we will use

Question 4

Formula for ligation

Answer 4

insert size/vector size * insert/vector= ng insert/ ng vector

Question 5

Ligation and transformation controls

Answer 5

look at chart