Neutrophils should be the predominant WBC in
the blood. How would you interpret a
neutrophil concentration of 80% in a dog?
That value must be interpreted in the context of the
[WBC]
What cell types are not reliably identified by an
automated hematology analyzer and indicates
that a manual WBC differential should be
performed?
Basophils, band neutrophils, nRBC
Basophils will count as a part of
[WBC]
band neut will be counted as
neutrophils
nRBC will be counted as
lymphocytes
What 2 values are used to calculate a
thrombocrit (AKA plateletcrit)?
MPV and [PLT]
How can you estimate platelet
concentration on a blood smear?
Count the average number of platelets per 100x
field and multiply by 15,000/uL
Quantitative Data of WBC in CBC
total concentration = specific WBC % and concentrations for neuts, lymphocytes, monos, eos, basos
Qualitative data of WBC/platelets
blood smear
Quantitative Data of platelets in CBC
Concentration, MPV, Pct
Thrombogram?
Platelet Evaluation
Platelet Concentration
of platelets per volume of blood
is [platelet] reliable if there’s platelet clump?
noooo
Hemocytometer?
rare platelet count method
MPV?
Mean Platelet Volume measured by automated..
How does storage affect MPV values?
Stored platelets (in lab samples or transfusion units) tend to swell, which falsely increases MPV
Pct?
Plateletcrit
Plateletcrit
% volume of blood filled by platelets
Why can’t analyzers directly measure Pct?
the value is too small to measure directly
How is Pct measured?
calculated from MPV and platelet concentration.
How does platelet size relate to function?
Larger platelets are stronger (more metabolically and enzymatically active) than smaller ones
What information can a blood smear provide about platelets?
for clumps, concentration estimate, size, inclusions
Leukogram?
WBC Evaluation
[WBC] measured by
automated hematology analyzer