lipid analysis

Question 1

non-destructible/reversible lipid analysis

Answer 1

iodine vapour; pretty unspecific dichlorofluorescin–>most nonspecific

Question 2

destructive/irr

Answer 2

ninhydrin–>detects for primary amine-containing lipids (PE and PS); phosphoric acid–>unspec, chars any organic material–>most specific

Question 3

gas chromatography of lipids requires adding a methyl ester

Answer 3

ya

Question 4

can also measure lipids in circulation via HPLC

Answer 4

similar to size exclusion chromatography–>important in generating a lipid profile