1
Q
EYA
A
- Egg Yolk Agar (EYA)
- Type: enriched, nonselective, and differential medium
- Purpose: presumptive differentiation of Clostridium spp. and other obligate anaerobes.
- Purpose: Based on lecithinase, lipase, and proteolytic activity
- Components: casein, yeast extract, dextrose, and tryptophan. Hemin and vitamin K1 have been included to improve the growth of anaerobes
- Appearance: iridescent sheen (“oil on water”) on the surface of the colony is observed by lipase-positive organisms, due to the breakdown of free fats present in the egg yolk.
- Appearance: Clearing of the medium surrounding the colonies as a result of proteolytic organisms.
- Appearance: lipase opaque precipitation under agar
- Conditions:
- QC: Positive Organism - Fusobacterium necrophorum Lipase +
- Clostridium perfringens - Lecithinase +
-QC: Negative Organism - Grows most organisms
2
Q
CMGS
A
- Cooked Meat Glucose Starch Broth
- Type: enrichment and differential
- Purpose: medium for the enrichment and cultivation of anaerobic microorganisms, particularly obligate anaerobes. The differential in showing proteolytic action.
- Components: Solid meat cubes are digested by proteolytic bacteria
- Appearance: Turbidity around meat cubes
- Conditions: Incubate uninoculated representative tubes at 20 – 25 °C and 30 – 35 °C and examine after 7 days for microbial contamination. Anaerobically
-QC: Positive Organism - *Clostridium perfringens-Moderate to heavy growth
*Porphyromonas levii - Moderate to heavy growth
-QC: Negative Organism -
3
Q
TPGY
A
- Tryptone Peptone Glucose Yeast Extract Broth Base w/o Trypsin
- Type: Selective/Enrichment for toxins
- Purpose: is used to test toxicity type of Clostridium botulinum cultures for types A-G, Trypsin activates non-proteolytic toxin types so that can be added later to test toxin.
- Components: Tryptone Peptone Glucose Yeast Extract w/o trypsin
- Appearance: Strong Luxuriant growth
- Conditions: Cultural characteristics observed under anaerobic conditions, after incubation at 26-28°C for up to 7 days.
- QC: Positive Organism - Clostridium botulinum
- QC: Negative Organism -
4
Q
Thayer–Martin agar
A
- Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a Mueller–Hinton agar with 5% chocolate sheep blood and antibiotics.
- Type: Selective
- Purpose: culturing and primarily isolating pathogenic Neisseria bacteria, including Neisseria gonorrhoeae and Neisseria meningitidis
- Components: Vancomycin, is able to kill most Gram-positive organisms, although some Gram-positive organisms such as Lactobacillus and Pediococcus are intrinsically resistant
- Components: Polymyxin, also known as colistin, is added to kill most Gram-negative organisms except Neisseria, although some other Gram-negative organisms such as Legionella are also resistant
- Components: Nystatin, which can kill most fungi
- Components: Trimethoprim inhibits swarming of Proteus spp
- Appearance: Small opaque, grayish-white to colorless, raised, glistening and smooth colonies are seen.
- Conditions: CO2-enriched (3%- 10%) environment and incubated at 35º-37ºC.
- QC: Positive Organism - Neisseria gonorrhea
- QC: Negative Organism - Escherichia coli
5
Q
Bismuth Sulfite Agar
A
- Type: selective and differential medium
- Purpose: Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide is differential for salmonella from E. coli and Shigella
- Components: Brilliant Green and bismuth selective against gram-positive growth
- Appearance: Typical S. Typhi surface colonies are olive-green to black, surrounded by black or brown-black zone with or without a metallic sheen.
- Conditions: 35 ± 2°C after 40 - 48 hours
incubation. - QC: Positive Organism - Salmonella spp.
- QC: Negative Organism - Escherichia coli
6
Q
Simmons’ citrate agar
A
- Type: selective and differential medium
- Purpose: differentiating gram-negative bacteria on the basis of citrate utilization by selecting for organisms that use citrate.
- Purpose: that tests for an organism’s ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.
- Components: bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9 and then turns blue at a pH of 7.6 or greater.
- Components: contains sodium chloride, sodium citrate, ammonium dihydrogen phosphate, dipotassium phosphate, and magnesium sulfate. bromothymol blue
- Appearance: – citrate utilization = produces an alkaline reaction = the color of the medium changes from green to bright blue.
- Appearance: – no citrate utilization = the color of the medium remains unchanged(green)
- Conditions: 24–48 h or up to 4 days at 35 ± 2 °C in aerobic atmosphere.
- QC: Positive Organism - KLEBSIELLA,
- Positive Growth: Serratia, and the majority of the Enterobacter, Citrobacter, Klebsiella, Proteus, and Providencia species
- QC: Negative Organism - E. COLI,
- Negative Growth: Shigella, Yersinia, Edwardsiella species
7
Q
Bile Esculin Agar (BEA)
A
- Type: selective and differential
- Purpose: Bile Esculin Agar is used primarily to differentiate Enterococcus from Streptococcus
- Components: Bile salts are the selective ingredient, while esculin is the differential component. -
- Components: Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium.
- Appearance: Brownish colonies with blackening of the medium
- Conditions: 24hr @ 35 ± 2 °C in an aerobic atmosphere.
- QC: Positive Organism - Enterococci
- QC: Negative Organism - S. pyogenes
8
Q
Motility GI Medium
A
- Type: differential
- Purpose: Differential organisms on the basis of motility
- Components: Standard agar sometimes with a dye
- Appearance: Motile organisms diffuse growth away from the line or spot of inoculation
- Appearance: Nonmotile organisms grow only along the line of inoculation.
- Conditions: Incubate at a temperature and duration appropriate for the suspected organism being tested.
- Positive organsim - Proteus mirabilis
- Negative Organism -Klebsiella pneumoniae
9
Q
CN/CIN AGAR
A
-Type: Selective
- Purpose: isolation and enumeration of Yersinia
spp. from food. - Components: sodium desoxycholate, crystal violet, cefsulodin, irgasan, and novobiocin, mannitol,
- Appearance: Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance.
- Appearance: The ratio of the transparent border to red center varies with serotype and environmental strains may appear rough with an irregular edge. Red Colonies
- Conditions: incubated at 30 ± 1C for 18-24 hours.
- QC: Positive Organism - Yersinia enterocolitica
- QC: Negative Organism - E. coli, Enterococci
10
Q
GN BROTH
A
Gram-Negative Broth
- Type: Enrichment and Selective
- Purpose: cultivation of gram-negative enteric organisms.
- Purpose: enrichment medium for the recovery of Salmonella and Shigella from clinical specimens.
- Components: Mannitol and dextrose are sources of energy.
- Components: Sodium citrate and sodium desoxycholate are added to inhibit gram-positive and some gram-negative bacteria
- Components: Mannitol is provided in a higher concentration than dextrose
- Appearance: Growth in broth media is indicated by turbidity compared to an uninoculated control
- Conditions: Incubate the tubes with loosened caps at 35 °C and subculture onto selective and differential media after 6–8 h of incubation and again after 18–24 h of incubation.
- QC: Positive Organism - Escherichia coli, Salmonella
- QC: Negative Organism - Gram Positive
11
Q
(HE) agar
A
Hektoen enteric
- Black Colonies for Salmonella
- Positive organsim - Salmonella, Shigella
- Negative Organism- Escherichia coli, Enterococcus faecalis, Gram-Pos
- Type: selective and differential agar
- Purpose: inhibiting Gram-positive organisms and reducing the growth of some gram-negative organisms
- Purpose: isolation of Shigella and Salmonella.
- Components: bile salts
- Components: bromothymol blue, acid fuchsin, and ferric iron as an indicator of the formation of hydrogen sulfide from thiosulfate.
- Appearance: -Blue color change Peptone utilization by Shigella and Salmonella
- Appearance: Black Colonies for Salmonella by the formation of hydrogen sulfide from thiosulfate.
- Appearance: Sugar fermentation changes color red everything else
- Conditions: - Incubate @ 35° for 18 to 48 hrs
- QC: Positive Organism - Salmonella
- QC: Positive differential Organism: Shigella not black colonies
- QC: Negative Organism - Escherichia coli, Enterococcus faecalis, Gram-Pos
12
Q
(XLD) agar
A
xylose-lysine-deoxycholate agar
- Type: selective and differential
- Purpose: isolation of Salmonella and Shigella
- Components: indicator phenol red, xylose, sucrose, lactose, deoxycholate
- Appearance: Salmonella can ferment the sugar xylose to produce acid which turns indicator yellow
- Appearance: Salmonellae metabolize thiosulfate to produce hydrogen sulfide to produce red colonies with black centers
- Appearance: Shigella colonies cannot do this and therefore remain red at neutral pH
- Conditions: Incubate @ 35° for 18 to 24 hrs
- QC: Positive Organism - Salmonella, Shigella
- QC: Negative Organism - Escherichia coli, Enterococcus faecalis, Gram-Pos
13
Q
(SS) agar
A
Salmonella-Shigella Agar
- Type: moderately selective and differential medium
- Purpose: isolation, cultivation, and differentiation of Salmonella spp. and some strains of Shigella spp.
- Components: Bile Salts, Sodium Citrate and Brilliant Green serve to inhibit gram-positive, coliform organisms and inhibit swarming Proteus spp
- Components: Thiosulfate and Ferric Citrate permit detection of hydrogen sulfide by the production of colonies with black centers. Neutral Red. lactose
- Appearance: Neutral red turns red in the presence of an acidic pH, thus showing fermentation has occurred.
- Appearance: Salmonella will not ferment lactose but produce hydrogen sulfide (H2S) gas. The resulting bacterial colonies will appear colorless with black centers.
- Appearance: Shigella do not ferment lactose or produce hydrogen sulfide gas, so the resulting colonies will be colorless.
- Appearance: E. coli produces pink to red colonies and may have some bile precipitation.
-Conditions: incubated aerobically at 37 ± 1°C for
18 - 48 hours
- QC: Positive Organism - Salmonella enteriditis = Colorless colonies with black center
- QC: Positive Organism - Shigella flexneri = Colorless colonies
- QC: Negative Organism - Escherichia coli
- QC: Negative Organism - Enterococcus faecalis
14
Q
(SEL) F broth
A
Selenite Broth
- Type: selective enrichment
- Purpose: isolation of Shigella, Salmonella, and other Enterobacteriaceae.
- Components: Sodium selenite inhibits many species of gram-positive and gram-negative bacteria including enterococci and coliforms.
- Appearance: Colorless, Good Growth.
- Appearance: pink with bile precipitate, Inhibited or no growth.
- Conditions: incubated aerobically at 35 degrees for 8–12 h incubation
- QC: Positive Organism - Salmonella Typhimurium
- QC: Negative Organism - Escherichia coli
15
Q
(SBA) or BA
A
Sheep Blood Agar
- Type: Nutrient and differential
- Purpose: General growth medium with differentiates the organism’s ability to hemolyze RBC
- Components: 5% Sheep Blood Agar
- Appearance: Beta-hemolysis complete clearing of red agar
- Appearance: a-hemolytic is Partial hemolysis incomplete clearing sometimes green
- Appearance: no hemolysis occurs, this is termed gamma-hemolysis.
- Appearance: colonies of all shapes and sizes
- Conditions: 35 degrees for 18 to 24 hrs
- QC: Positive Organism - Grows almost all organisms
- QC: Negative Organism - Differential in hemolysis organisms
16
Q
(MSA)
A
Mannitol Salt Agar
- Type: Selective and differential
- Purpose: It is used for the selective isolation and differentiation of Staphylococcus aureus from clinical samples.
- Components: 7.5% concentration of sodium chloride and mannitol with phenol red
- Appearance: phenol red color change due to fermentation creating acid
- Conditions: 35 degrees for 18 to 24 hrs
- QC: Positive Organism - Staphylococcus Aureus
- QC: Negative Organism -
17
Q
Chrom Agar
A
- Type: Differential
- Purpose: Direct differentiation of certain species or the detection of certain groups of organisms with only a minimum of confirmatory tests.
- Components: a chromogen mix that consists of artificial substrates
(chromogens) that release differently colored compounds upon degradation by specific microbial enzymes - Appearance: Different for each organism that the media is made for
- Conditions: 35 degrees for 18 to 24 hrs
- QC: Positive Organism - Varies
- QC: Negative Organism - Varies
18
Q
(CNA)
A
Columbia Agar
- Type: Selective
- Purpose: growth of staphylococci, hemolytic streptococci, and enterococci while inhibiting the growth of Proteus, Klebsiella, and Pseudomonas specie
- Components: -5% sheep’s blood, Colistin, Nalidixic Acid
- Appearance: Normal colony Growth
- Conditions: 35 degrees for 18 to 24 hrs
- QC: Positive Organism - staphylococci
- QC: Negative Organism - Klebsiella
19
Q
(PEA) agar
A
- Phenyl-ethyl alcohol
- Type: Selective
- Purpose: selective for Gram-positive organisms, particularly cocci, from a sample containing a mixture of pathogens
- Purpose: an enriched selective medium used for the inhibition of facultative gram-negative rods and to inhibit the swarming of certain Clostridium species and Proteus species from clinical specimens.
- Components: The active ingredient, phenyl ethyl alcohol, inhibits or markedly reduces the growth of Gram-negative organisms by interfering with DNA synthesis.
- Appearance: Normal colony Growth
- Conditions: 35 degrees for 18 to 24 hrs usually in anaerobic conditions
- QC: Positive Organism - Gram-Positive
- QC: Negative Organism - Escherichia coli
20
Q
(MAC)
A
- MacConkey Agar
- Type: selective and differential medium
- Purpose: isolate and differentiate enterics based on their ability to ferment lactose.
- Components: Bile salts and crystal violet inhibit the growth of Gram-positive organisms. Neutral red
- Components: Lactose provides a source of fermentable carbohydrates, allowing for differentiation.
- QC: Positive Organism - Escherichia coli for lactose fermentation
- QC: Positive Organism - Pseudomonas aeruginosa for non- lactose fermentation
- QC: Negative Organism - Streptococcus pneumoniae
21
Q
(CHOC)
A
- Chocolate Agar
- Type: Nutrient
- Purpose: Fastidious organisms like Haemophilus influenzae, Neisseria gonorrhoeae, and other fastidious aerobes and anaerobes in the correct environment.
- Components: boiled blood results in the release of cellular contents of RBC
- Components: cofactor V (nicotinamide adenine dinucleotide; NAD) and cofactor X (hemin)
- Purpose: growth, when incubated in 5% – 10% CO2, is not obligate anaerobes
- Appearance: Normal Colony growth
- Conditions: Incubate @ 35° for 18 to 48 hrs
- Conditions: Incubate @ 5% – 10% CO2
- QC: Grows most organism
- QC: Positive Organism = Haemophilus influenza
- QC: Negative Organism = Neisseria gonorrhea in anaerobic conditions
- QC: Negative Organism = Fusobacterium necrophorum in 5% CO2
22
Q
Brilliant Green Agar
A
- Type: selective and differentiation medium
- Purpose: isolation of salmonellae other than S. Typhi from feces and other clinical specimens
- Components: yeast extract and two peptones provide the nutrients
- Components: lactose and sucrose together with phenol red provide a differentiation system to exclude lactose and/or sucrose fermenters (e.g. E. coli) while salmonellae do not produce acid from these sugars.
- Components: Brilliant Green is the selective agent to inhibit the accompanying flora. phenol red.
- Appearance: White to red colonies surrounded by red zones = Salmonella
- Appearance: Yellow to greenish colonies surrounded by yellow-greenish zones = Escherichia coli
- Conditions: 35 to 37° C for 18 to 48 hours.
- QC: Positive Organism - Salmonella (other than S. Typhi and S. Paratyphi)
- QC: Negative Organism - Gram-positive bacteria
- QC: Selective organism - Escherichia coli, Klebsiella and Enterobacter
23
Q
CT-SMAC
A
- MacConkey Sorbitol Agar w/ Cefixime and Tellurite
- Type: selective and differential
- Purpose: the detection of Escherichia coli serotype O157:H7
- Components: Sorbitol MacConkey II Agar with Cefixime and Tellurite (SMAC-CT)
- Appearance: while sorbitol non-fermenters produce colorless colonies. = 0157
- Appearance: Colored colonies not 0157
- Conditions: 35 ± 2°C for 18 – 24 h
- QC: Positive Organism - Escherichia coli serotype O157:H7 - Growth. colorless colonies
- QC: Negative Organism - Any other E. coli, no growth or pink colonies
24
Q
Tetrathionate Broth
A
- Tetrathionate Broth Base
- Type: selective enrichment medium
- Purpose: with added iodine-iodide solution, is used as a selective enrichment medium for the isolation of Salmonella from feces, urine, foods, and other materials of sanitary importance.
- Purpose: Bile salts inhibit gram-positive microorganisms. Tetrathionate, which is formed in the medium by the addition of the iodine-iodide solution, inhibits the normal intestinal flora of fecal specimens.
- Components: Bile salts, Tetrathionate from iodine-iodide solution.
- Conditions: 35 ± 2 °C for 18 – 24 h in an aerobic atmosphere
- Appearance:
- QC: Positive Organism - S. Typhimurium
- QC: Negative Organism -
25
(EMB)
Eosin methylene blue agar
- Type: selective and differential
- Purpose: isolate fecal coliforms. Differentiate culture of Salmonella and Shigella
- Principle: Eosin Y and methylene blue are pH indicator dyes that combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram-positive organisms. Sucrose and lactose serve as fermentable carbohydrate sources which encourage the growth of fecal coliforms and provide a means of differentiating them.
- Components: Eosin Y, methylene blue, Sucrose, lactose
- Conditions: 35 ± 2 °C for 18 – 24 h in an aerobic atmosphere
- Appearance: Purple colonies for fermenters
- QC: Positive Organism - E.coli - Good growth, blue-black colonies with a green metallic sheen
- QC: Negative Organism - Gram-positive organisms
26
Thio Sulfate Citrate Bile Sucrose
- Type: Selective and differential
- Purpose: TCBS Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios
- Principle: Thiosulfate and sodium citrate, as well as the alkalinity of the medium, considerably inhibit the growth of Enterobacteria. Ox bile and sodium cholate slow the growth of enterococci and inhibit the development of Gram-positive bacteria. The acidification of the medium resulting from the fermentation of sucrose by Vibrio makes bromthymol blue turn yellow. Bromthymol Blue and Thymol Blue are pH indicators. Using thiosulfate as a sulfur source, the production of hydrogen sulfide is visualized in the presence of ferric citrate.
- Components: Thiosulfate and sodium citrate, Ox bile and sodium cholate, Bromthymol Blue and Thymol Blue, sucrose
- Conditions: 35 ± 2 °C for 18 – 24 h in an aerobic atmosphere
- Appearance: Yellow colonies = Vibro cholera, Black colonies = parahaemolyticus
- QC: Positive Organism - Vibrio parahaemolyticus
- QC: Negative Organism - Enterococcus faecalis
27
Skirrow's Agar
- Type: selective media
- Purpose: for the isolation of Campylobacter species from clinical and other specimens.
- Principle: In BD Campylobacter Agar (Skirrow), heart infusion, casein peptone, and yeast extract provide nutrients, and sodium chloride maintains the osmotic stability. Vancomycin inhibits Gram positives, and trimethoprim and polymyxin B inhibit many Gram-negative organisms. Lysed horse blood provides nutrients and heme for bacterial catalase.
- Components: Vancomycin trimethoprim polymyxin B
- Conditions: 35 to 37° C for 42 to 48 hours.
- Appearance: Strong colony Strange looking colony
- QC: Positive Organism - Campylobacter jejuni
- QC: Negative Organism - Escherichia coli
28
Alkaline Peptone Water
- Type: enrichment
- Purpose: enrichment of Vibrio species formulated peptone water for use as a nonselective enrichment broth for the cultivation of Enterobacteriaceae from foodstuffs and other specimens.
- Principle:The alkaline pH of this medium allows the growth of Vibrio organisms while inhibiting the growth of commensal intestinal bacteria. Enrichment of specimens in Alkaline Peptone Water prior to inoculation of plated media has been shown to increase the recovery rate of Vibrio spp.
- Components: Peptone, NaCl, pH= 8.6
- Conditions: incubated at 35-37°C for 5-6 hours or 18-20 hours at 18-20°C.
- Appearance: Turbidity
- QC: Positive Organism - Vibrios
- QC: Negative Organism -
29
CAMPY agar
- Type: selective swabs.
- Purpose: isolation of Campylobacter jejuni subsp. jejuni from fecal or rectal
- Principle:CAMPY is an enriched selective blood agar that will support good growth of Campylobacter jejuni subsp. jejuni. Based off Skirrow’s formulation, Brucella Blood Agar (BRU) is used as the nutritive base and is supplemented with trimethoprim, vancomycin, and polymyxin B to inhibit normal enteric bacteria.
- Components:trimethoprim, vancomycin, and polymyxin B, Blood agar
- Conditions: 35-37°C for 18-24hrs
- Appearance: Growth
- QC: Positive Organism - Campylobacter jejuni
- QC: Negative Organism - Normal bowel flora organisms such as Proteus mirabilis, Escherichia coli, Enterococci spp., and Clostridium perfringens should be inhibited.
30
Phosphate Buffered Saline
- Type: enrichment broth
- Purpose: Enrichment of Y. enterocolitica
- Principle: inoculated then refrigerate @ 2-8 degrees for up to 21 days. Plate onto media every few days
- Components: phosphate-buffered saline
- Conditions: @ 2-8 degrees for up to 21 days.
- Appearance:
- QC: Positive Organism - Y. enterocolitica
- QC: Negative Organism -
31
Lauryl Tryptose Broth
- Type: selective medium
- Purpose: the detection of coliform bacteria in water and wastewater.
- Principle: Lactose fermenters produce gas and acid. Presumptive Media
- Components: Sodium lauryl sulfate = selective agent for the inhibition of non-coliform organisms, Tryptose, Lactose
- Conditions: 35°C for 48 hrs
- Appearance: Turbidity, Gas Production
- QC: Positive Organism - E. coli, Klebsiella
- QC: Negative Organism - S. Aureus
32
Brilliant Green Broth
- Type: Selective
- Purpose: Brilliant Green Bile Broth 2% is used for the detection of coliform bacteria in water, food, and dairy products in a laboratory setting.
- Principle: Confirmatory Medium for Total Coliforms. BG and Ox Bile are selective against non-coliforms. Proceeded by LTB.
- Components: Lactose, Ox Bile, Brilliant Green
- Conditions: 35°C for 48 hrs
- Appearance: Turbidity, Gas Production
- QC: Positive Organism - E. coli, Klebsiella
- QC: Negative Organism - S. Aureus, E. faecalis
33
EC Broth
- Type: Selective
- Purpose: the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C.
- Principle: Heat selection @ 44.5 and Bile salts for gram pos.
- Components: Tryptone, Lactose, Bile Salts
- Conditions: 44.5°C for 24 hrs
- Appearance: Gas and turbidity
- QC: Positive Organism - E. coli
- QC: Negative Organism - K. aerogenes
34
Colilert
- Type: differential
- Purpose: Defined Substrate Technology (DST) to simultaneously detect total coliforms and E. coli.
- Principle: Two nutrient-indicators, ONPG and MUG, are the major sources of carbon in Colilert and can be metabolized by the coliform enzyme β-galactosidase and the E. coli enzyme β-glucuronidase, respectively.
- Components: ONPG = O-nitrophenyl to O-nitrophenol, MUG = 4-methyl-umbelliferyl to 4-methyl-umbelliferone
- Conditions: 35.0°C for 24-28 hrs
- Appearance: Yellow for Total, Fluorescent for E. coli
- QC: Positive Organism - E. coli, Klebsiella
- QC: Negative Organism - P. aeruginosa
35
Enterolert
- Type: differential
- Purpose: Defined Substrate Technology (DST) nutrient indicator to detect enterococci.
- Principle: β-D-glucosidase with 4-methyl-umbelliferyl can be metabolized by the Enterococci enzyme Glucosidase to produce 4-methyl-umbelliferone
- Components: β-D-glucosidase with 4-methyl-umbelliferyl
- Conditions: 41°C for 24-28 hrs
- Appearance: Fluorescent for Enterococci
- QC: Positive Organism - E. faecalis
- QC: Negative Organism - E. coli
36
BCYE
- Type: differential and selective
- Purpose: BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples
- Principle:
- Components: Buffered charcoal yeast agar
-Conditions: incubate at 36±1°C for 7 to 10 days
-Appearance: small to large, smooth, colorless to pale, blue-grey, slightly mucoid
colonies that fluoresce yellow-green under longwave UV light.
- QC: Positive Organism - Legionella
- QC: Negative Organism -
Microbiology
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