1
Q
Presence/absence (SM 9223 B-c) steps
A
- Make sure you have proper PPE on (lab coat, goggles, gloves)
- Get samples
- Prep the logbook
- Sterilize your work area and gloves
- Inspect samples (check for damage/leaks and volume)
- Label the sample bottles
- Add the media packets
- Prep the QCs
- Colilert 18 goes in the water bath at 44.5 +/- 0.2°C for 7-10 minutes to activate the media then the incubator at 35 +/- 0.5°C for 18-22 hours
- Colilert and Colisure go directly in the incubator at 35 +/- 0.5°C for 24-28 hours (Colisure can go up to 48 hours if needed)
- At the reading time, take the samples out of the incubator and read the QCs first then use the comparator with the samples
2
Q
What bacteria does P/A test for?
A
Total coliforms and E. coli
3
Q
What are the QCs for P/A?
A
+/+: E. coli
+/-: K. pneumoniae
-/-: P. aeruginosa
4
Q
Quanti-tray (SM 9223 B-b) steps
A
- Make sure you have proper PPE on (lab coat, goggles, gloves)
- Get samples
- Prep the logbook
- Sterilize your work area and gloves
- Inspect samples (check for damage/leaks and volume)
- Label the sample bottles
- Add the media packets (sea water and fecal only Colilert 18)
- Turn on QT sealer
- Label packets while sealer is heating up
- When sealer is ready, pour samples into packets one at a time and run through sealer
- Incubate samples (Colilert 18 at 35 +/- 0.5°C for 18-22 hours, fecal goes in the water bath at 44.5 +/- 0.2°C for 18-22 hours, Colilert and Colisure at 35 +/- 0.5°C for 24-28 hours)
5
Q
What bacteria does Quanti-tray test for?
A
Total coliforms
E. coli
Fecal coliforms
6
Q
What are the QCs for Quanti-tray?
A
+/+: E. coli
+/-: K. pneumoniae
-/-: P. aeruginosa
7
Q
HPC (SM 9215 B) steps
A
- Make sure you have proper PPE on (lab coat, goggles, gloves)
- Get samples
- Prep the logbook
- Sterilize your work area and gloves
- Inspect samples (check for damage/leaks and volume)
- Label the sample bottles
- Turn off the AC
- Set up plates and label them according to the sample bottle labels
- Shake each sample 25 times and pipette 1 mL onto each plate
- Open melted agar bottle and sterilize rim and cap in Bunsen burner flame
- Pipette 10-12 mL of agar onto each plate and swirl as you go
- Weight the media plate and record the initial weight
- Let the agar solidify, invert the plates, and incubate them at 35 +/- 0.5°C for 48 +/- 3 hours
- Take the plates out at the reading time and weigh the media plate
