Micro lab Practical #1

Question 1

Gram Stain

Answer 1

Primary: Crystal Violet
Mordant: Gram’s Iodine
Decolorizer: Ethanol
Secondary: Safranin

Question 2

Gram + Example

Answer 2

Staphylococcus or S. aureus or B. megaterium

Question 3

Gram - Example

Answer 3

E. coli

Question 4

Capsule Stain

Answer 4

Primary: Crystal Violet
Counterstain: Copper Sulfate
NO HEAT FIX NO RINSE

Question 5

Capsule Stain Example

Answer 5

Flavobacterium

Question 6

Acid Fast

Answer 6

mycolic acid in cell wall
Primary: carbol fuchsin
Decolorizer: Acid Alcohol
Counter Stain: Methyl Blue

Question 7

Endospore Stain

Answer 7

Primary: Malachite Green
Decolorizer: Water
Counter Stain: Safranin

Question 8

Psychrophile

Answer 8

-5-20 degrees Celcius

Question 9

Mesophile

Answer 9

20-45

Question 10

psychrotroph

Answer 10

mesophile range to below 10 degrees

Question 11

thermophile

Answer 11

45-80

Question 12

hyperthermophile

Answer 12

80-105

Question 13

BHI

Answer 13

brain heart infusion, used to determine oxygen classification

Question 14

anaerobic Broth

Answer 14

thioglycolate broth, contains sodium thioglycolate

Question 15

MSA

Answer 15

mannitol salt agar, contains: 7.4% NaCl, mannitol, and phenol red
selective for halotolerant
ferment=yellow
staphylococcus

Question 16

Blood Agar

Answer 16

Detect hemolysis and what type

Question 17

beta hemolysis

Answer 17

complete lysis, clearing around bacteria

Question 18

alpha hemolysis

Answer 18

partial lysis, grayish-green

Question 19

gamma hemolysis

Answer 19

no lysis

Question 20

MacConkey

Answer 20

selects for gram - bacteria based on the ability to ferment lactose (pinkish red colony)
and differential
contains crystal violet and bile salts

Question 21

EMB

Answer 21

Eosin Methyl Blue
contains eosin and methyl blue
selects for gra negative
differential between lactose fermenting organisms (pinkish colonies)

Question 22

CCNA

Answer 22

Columbia C CNA,
contains antibiotics: colistin and nalidixic acid
selective for gram+
ex: staphylococcus

Question 23

Mueller Hinton Plate

Answer 23

tests chemicals on microorganisms using disk diffusion assay

Question 24

E Test

Answer 24

epsilometer
measures minimum inhibitory concentration (using concentration gradient)

Question 25

additive affect

Answer 25

no greater than the sum

Question 26

synergistic

Answer 26

greater than the sum (if merge in the middle)

Question 27

Snyder Test agar

Answer 27

determine a person’s susceptibility to dental caries based on acid production contains: glucose and bromcresol green green= slight susceptibility yellow= high

Question 28

Testing throat and mouth cultures

Answer 28

MSA, Mueller Hinton, Chocolate Agar