- For virus indication into cell culture a method is used that based onto changes of pH of culture
medium during cultivation viruses in vitro. What is the name of this virus indication method?
1/Color test 2/Cytopathic effect 3/Hemagglutination test 4/Hemadsorption 5/Plaque formation
Color test
*For vaccine preparation microbial cultures are propagated onto artificial nutrient media. Which
microorganisms do not grow into artificial nutrient media?
Viruses Fungi Actynomyces Protozoa Micoplasma
Viruses
*A sample from patient with diagnosis “Influenza” was sent to laboratory. During virological
investigation hemadsorption test has been used. Which viruses can be revealed with this test?
All of naked viruses All of enveloped viruses Viruses possessing hemagglutinins on their surface DNA-including viruses Any virus
Viruses possessing hemagglutinins on their surface
- A sample from patient with diagnosis “ARVI” was sent to laboratory for virological
investigation. What is purpose of virus typing isolated from patient’s sample?
Etiological diagnostics of virus infection
To study biological properties of viruses
To study physico-chemical properties of viruses
Development of non-specific prevention measures
To study virus resistance to environmental factors action
Etiological diagnostics of virus infection
- During virological investigation of a sample, collected from patient A., 15 year old, with
suspicious infectious mononucleosis, Epstein-Barr virus (EBV) was revealed. Which order of
DNA-including viruses replication into the host cell is correct?
Deproteinization, transcription, protein synthesis, virus DNA synthesis
Deproteinization, protein synthesis, transcription, virus DNA synthesis
Transcription, protein synthesis, deproteinization, virus DNA synthesis
Protein synthesis, deproteinization, transcription,, virus DNA synthesis
No one
Deproteinization, transcription, protein synthesis, virus DNA synthesis
- Papilloma virus, the 16th type, was revealed with PCR in a patient with cervical malignancy. It is
known, this virus integrates own DNA with host genome. What is the name of virus integrated with
cell genome?
Provirus Viroid Virusoid Virion Prion
Provirus
*A child, 1.5 year-old, had been suspected with adenovirus infection by physician. With CFT
antibody titer to adenovirus in patient serum was revealed as 1:20. During recovery (after 2 weeks)
serology was repeated. Which result confirms previous diagnosis?
Decreasing of antibody titer Increasing of antibody titer The same titer of antibody Negative result of CFT Uncomplete antibody revealing
Increasing of antibody titer
- A district doctor recommended interferone usage to prevent influenza. What is mechanism of
action of this preparation?
Damage maturation of a virus
Inhibition of virus releasing from cell
Blockage of translation initiation and destruction virus-specific m-RNA
Blockage of virus deproteinization
Inhibition of virus adsorption onto cell receptors
Blockage of translation initiation and destruction
*To confirm diagnosis of acute respiratory virus infection with laboratory methods a doctor decided
to use serological method of diagnostics. Which way should material be collected for this
investigation?
At onset of disease (as early as possible) and after 2-3 weeks
At onset of disease (as early as possible)
At the end of disease, in period of symptoms fading
At peak of disease, when all clinical symptoms are bright
At maximal data of body temperature
At onset of disease (as early as possible) and after 2-3 weeks
- For prevention of post-surgery complication abdominal cavity of patient was inoculated with 50 ml of liquid polyvalent staphylococcal phage. What is mechanism of this drug action?
Lyses of microbial cells Neutralization of staphylococcal toxins Immunity activation Inhibition of causative agent growth Disorder of virulent enzymes biosynthesis
Lyses of microbial cells
A child, 8 month old, has intestinal disorders. Qualitative and quantitative assay of intestinal
microbiota revealed disbiosis. Coli-proteus bacteriophage is administered for treatment. What is
mechanism of this drug action?
Cause lyses of opportunistic enterobacteria
Promotion multiplication of Bifidibacteria
Enhance antagonistic effect of Lactobacilli
Stimulate synthesis of secretory IgA
Intencify barrier properties of intestinal mucous membrane
Cause lyses of opportunistic enterobacteria
- For gene-ingeneering insulin production a strain of E.coli was created, that includes gene
determining synthesis of this hormone. It is the most reasonable in strain-producer creation to use
vector which causes destruction of E.coli cell after task is made. Which kind of genetic structure is
this?
Plasmids Bacteriophage Isolated DNA-fragment Isolated RNA-fragment Transposon
Bacteriophage
It is known that human immunodeficiency virus belongs to Retroviridae family. What
basic feature characterizes this family?
Simple viruses, which affect only human beings.
Contain RNA of negative polarity.
Presence of reverse transcriptase enzyme.
Nucleic acid does not integrate into the genome of the host.
DNA-containing viruses which cause chronic infections.
Presence of reverse transcriptase enzyme.
It was revealed that T-lymphocytes were affected by HIV. Virus enzyme – reverse
transcriptase (RNA-dependent DNA-polymerase) – catalyze the synthesis of:
A.Viral DNA on DNA matrix B. DNA on virus ribosomal RNA C. Virus informational RNA (m-RNA) on the matrix of DNA D.mRNA on the matrix of virus protein E. DNA on the matrix of virus mRNA
DNA on the matrix of virus mRNA
Rather often the cause of acquired immunodeficiency is the infectious damage of organism which is
characterized by causative agents’ reproduction directly in immune system cells and destruction of these
cells. Choose such disease among given below.
A. AIDS. B.Diphtheria. C.Poliomyelitis. D. Cholera. E.Epidemic typhus
AIDS
Quite often the cause of secondary immunodeficiency is an infection involvement, when
the causative agents propagate directly in the cells of immune system and destroy it. The
following diseases are characterized by:
A Infectious mononucleosis, AIDS B Tuberculosis, mycobacteriosis C Poliomyelitis, type A hepatitis D Dysentery, cholera E Q-fever, epidemic typhus
Infectious mononucleosis, AIDS
During autopsy there were dark-red spots, plaques and nodes (Kaposi’s sarcoma) on the skin
of distal part of low extremities. Acute pneumonia was also revealed, caused with Pneumocysts.
What disease are these changes typical for?
A. AIDS B. Anthrax C. Diphtheria D.Influenza E. Measles
AIDS
The most often causative agent of opportunistic pneumonia at HIV/AIDS infection is: A. Pneumocysts B. Staphylococci C. Streptococci D.Cytomegoloviruses E. klebsiella
Pneumocysts
*A patient, homosexual man, was admitted to hospital with complaints of general weakness, dry cough, and fever. Bilateral root infiltrates were indicated with X-ray examination. What is the most probable reason of such pneumonia? A. Pneumocysts B. Staphylococci C. Streptococci D.Mycoplasma E. Klebsiella
Pneumocysts
