Microbial techniques

Question 1

Aseptic technique

Answer 1

Prevents contamination of culture & environment.
Flame loop → lift lid minimally → sterilise bench → work near Bunsen (airbourne contaminents away).

Question 2

Inoculation methods

Answer 2

streak plate (isolates colonies)
spread plate (counting colonies)

Question 3

Incubation

Answer 3

Usually 25°C in schools (not 37°C) to prevent growth of human pathogens.

Question 4

Explain why aseptic technique is important

Answer 4

Contamination
Reliability
Safety