MICROSCOPE

Question 1

early 1600s microscope are:

Answer 1

von leeuwanhoek simple microscope and janssens compound microscope

Question 2

1670

Answer 2

hooks microscope

Question 3

1850

Answer 3

powell and lealand no.1

Question 4

1930

Answer 4

zeiss laboratory microscope

Question 5

this microscope uses visible light to generate images

Answer 5

light (optical) microscope

Question 6

what are the diff types of microscopes

Answer 6

• simple
• compound
• stereo
• digital
• fluorescence
• inverted
• automated imaging system
• confocal
• super resolution microscopy
• imaging flow cytometry

Question 7

• magnification up to 300 times
• used to look at opaque objects
• provide close up 3d view of objects
• use in biological and medical science applications

Answer 7

Stereo (Dissecting) Microscope

Question 8

• use laser light to scan samples
• with aid of dichromatic mirror
• produce maginified image on computer screen

Answer 8

confocal microscope

Question 9

• uses electron rather than light
• sample are scanned in vacuum or near vacuum
• undergoing dehydration and then being coated

Answer 9

scanning electron microscope

Question 10

• uses electrons creating magnified image
• offers high degree of both magnification and resolution

Answer 10

Transmission Electron Microscope

Question 11

what part is used for adjustment and support?

Answer 11

Mechanical

Question 12

whats part is used for enlarging the specimen?

Answer 12

magnifying parts

Question 13

whats part is used for providing light?

Answer 13

illuminating parts

Question 14

• Bottommost portion that supports entire/lower microscope

Answer 14

Base

Question 15

part above the base supports other parts

Answer 15

pillar

Question 16

allows tilting of microscope for convenience of user

Answer 16

inclination joint

Question 17

curved/slanted part, held while carrying microscope

Answer 17

arm/neck

Question 18

attached to arm and bears lenses

Answer 18

body tube

Question 19

cylindrical structure on top of body tube holds ocular lenses

Answer 19

draw tube

Question 20

platform where object be examined is placed

Answer 20

stage

Question 21

secures specimen to stage

Answer 21

stage clips

Question 22

allows light to pass through specimen for better view

Answer 22

aperture

Question 23

also known as body, carries optical parts in upper part of microscope

Answer 23

head

Question 24

when turned, raises or lowers eyepiece in order to adjust the focus for each eye

Answer 24

diopter adjustment

Question 25

rotating disc where objective attached

Answer 25

revolving/ rotating nosepiece

Question 26

lies atop nosepiece and keeps dust from settling on objectives

Answer 26

dust shield

Question 27

geared to body tube which elevates or lowers when rotated bringing object into approx focus

Answer 27

coarse adjustment knob

Question 28

smaller knob for delicate focusing bringing object into perfect focus

Answer 28

fine adjustment knob

Question 29

elevates and lowers condenser

Answer 29

condenser adjustment knob

Question 30

lever in front of condenser and which moved horizontally to open/close diaphragm

Answer 30

Iris diaphragm lever

Question 31

gather wavefronts from microscopic light source and concentrate them into a cone of light

Answer 31

condenser

Question 32

controls the amount of light reaching specimen

Answer 32

iris diaphragm

Question 33

located beneath stage and has concave and plane surfaces to gather and direct light

Answer 33

mirror

Question 34

built in illuminator beneath stage that may be used if sunlight is not prefered

Answer 34

electric lamp

Question 35

another set of lens found on top of the body tube which function to further magnify the image produced by objective lenses

Answer 35

ocular/eyepiece

Question 36

metal cylinders attached below nosepiece

Answer 36

objectives

Question 37

objectives that gives lowest, 10x

Answer 37

Low power objective

Question 38

objective gives higher, 40x

Answer 38

high power objective

Question 39

objective gives highest magnification. 100x, used wet with cedar wood oil or synthetic oil

Answer 39

oil immersion objective

Question 40

provide observes with enough, 4x

Answer 40

scanner

Question 41

ability of lens to distinguish 2 objects that are close together

Answer 41

Resolution

Question 42

function of objective lenses and its ability to gather light

Answer 42

numerical aperture

Question 43

resolving power of compound microscope?

Answer 43

200 nanometer (nm)

Question 45

resolving power of transmission electron microscope?

Answer 45

0.2 nanometer

Question 46

resolving power of scanning electron microscope

Answer 46

10 nanometer

Question 47

degree of enlargement

Answer 47

magnification

Question 48

useful magnification and empty magnification

Answer 48

- useful; 500x-1000x - empty: 1400x and beyond

Question 49

refers to objectives that can be changed with minimal or no refocusing

Answer 49

parfocal

Question 50

determined by linear measurement of objective front lens to specimen

Answer 50

working distance

Question 51

- extent of observable area in distance units - area you see under microscope

Answer 51

Field of view

Question 52

used to measure minute distances or apparent diameters

Answer 52

micrometer microscope