Simple Microscope
-contains only a single lens responsible for enlarging the specimen
-have less resolution power and less magnifying power
-cannot distinguish between two objects
Compound Microscope
How to pick up a microscope
-one hand holding the base (support) and the other holding the arm (carry)
-the base and the arm provide a frame to support the entire microscope
Slide holder
-holds the slide in place
stage
-platform upon which the specimen is placed
-contains a center opening to allow light to hit the specimen (illumination exit)
X-Y stage control
-these control the movement of the slide in the stage clips, allowing you to position it over the light
Illumination Exit
light source from the bottom of the microscope
condenser
-lens below the stage that concentrates light from the light source into a narrow beam on the specimen
Iris diaphragm
-Shutter to the condenser that controls amount of light permitted to pass through the specimen
ocular
-used to look at specimen, eyepiece of the microscope houses the ocular lenses
-Has 10X magnifying power
Objectives
-lens placed closes to the object /specimen viewed attached to the rotating nose piece
-each objective lens has a different magnifying power
Scanning
4X - Red
Low Power
10x- Yellow
High Power
40X- Blue
Oil Immersion
100X- White
Coarse Adjustment
Moves the stage up and down at large increments to allow focus of the field of view
Fine Adjustment
-Moves the stage up and down at smaller increments to allow focusing of the field of view
Total Magnification
TM = objective lens x ocular lens
-it is the product of both magnifications
Prokaryotes
Eukaryotes
State the difference between prokaryotic and Eukaryotic cells
What features do all cells (prokaryotic and Eukaryotic) have in common