1
Q
Benefits
A
Rapid Results
2
Q
2 refractive lens
A
converging ()
Diverging ) (
3
Q
Bright field Principle+ diagnosis
A
stain required = non -viable tissue
Diagnoses cancers + infectious disease e_g Gonorrhoea.
4
Q
Dark field
A
uses diff refractive indices of cell component = increase contrast
Living tissues e.g Syphilis
5
Q
phase contrast
A
Enhances natural contrast (no dye)
2 extra components : annular+ phase ring e.g Gout
6
Q
Fluorescent
A
High contrast + specificity
Quantitative
Live cell imaging compatible
E.S TB
7
Q
Stokes shift
A
differences between absorption + emission wavelength
8
Q
Electron
A
Requires fixing
No live cell imaging
High resolution, contrast by post image colouring
Eg DNA transcription
