microscopy

Question 1

list the different types of microscopy

Answer 1

• electron microscopy
• dark field
• contrast
• bright field or light microscopy aka compound microscope

Question 2

What is the sequence of light passage in a compound microscope?

Answer 2

1-light source
2-condensor lenses
3-condensor diaphragm
4-speciman
5-objective
6- body
7-ocular
8-eye or camera

Question 3

Which objective is always used to start viewing your slide?

Answer 3

the 4X objective

Question 4

What function does immersion oil serve when viewing through the 100X objective?

Answer 4

• improves the optics because it unites the glass cover slip and the objective?
• oil has the same refractive index as glass, which means that the light bean will travel through glass and oil with minimal light scattering and refraction

Question 5

explain the term “parafocal”

Answer 5

the property by which when one of the lenses is focused on a specimen, the others should also be in focus when they are swung into place.