Microscopy

Question 1

What images form from a convex lens, when the object is outside/inside the focal point?

Answer 1

• inside: virtual upright image,no projection allowed.

- outside: real inverted image.

Question 2

Resolution

Answer 2

• ability to distinguish two things as separate entities.

- resolution= d= (0.61*wl)/(n sin a) minimize d for best resolution

Question 3

Microscopes used today. (5)

Answer 3

• compound: multiple lens stacked together.(dead, thin sample)
• phase contrast: brightness adjusted by phase shifts.(live sample)
• fluorescence: detect cells with UV activation
• confocal: generate 3-D. increases resolution(produces thin sliced images)
• TEM: use of electron beam, image on screen

Question 4

Refraction

Answer 4

-bending of light as it passes through mediums.
- velocity slows, wavelength is changed.
measured in diopters