Microscopy

Question 1

What are the 5 main components of a microscope?

Answer 1

• light source
• detector
• stage to hold the sample
• objective lens
• light-conditioning system

Question 2

What 2 parameters must be controlled in live imaging?

Answer 2

Carbon dioxide and Temperature

• live samples will want to be in their optimum condition
• small variations can change the plane of the stand, stage and objective - changing the focus

Question 3

Which microscopy technique is related to Stoke’s shift?

Answer 3

Fluorescence

- the difference between the wavelength absorbed vs emitted

Question 4

Magnification vs numerical aperture

Answer 4

Magnification = the capability of a specific objective to increase the size of the specimen when observed
NA = degree of definition an objective can give us

Question 5

What is confocal microscopy?

Answer 5

• removes out of focus light by adding a spatial pinhole in the confocal plance

Question 6

Advantages and disadvantages of confocal microscopy

Answer 6

• Higher Z-resolution and reduced out-of-focus blur

- Only a thin portion of the sample can be imaged at once