Microscopy

Question 1

What is a light microscope

Answer 1

Uses and light and lenses to form an imagine of a specimen and magnify it

Question 2

What is an electron microscope

Answer 2

Uses electrons to form an image. They have a much higher magnification and resolution, lets us see smaller things in detail

Question 3

What is the formula for magnification

Answer 3

Magnification= image size/real size

Remember image size and real size should have the same units

Question 4

How do you write small numbers

Answer 4

Standard form

Question 5

How do you prepare a slide

Answer 5

1. You need to put the specimen on a slide
2. Add a drop of water to middle of slide
3. Cut an onion and separate into layers. Use tweezers to peel off epidermal tissue.
4. Place into water on the slide
5. Add a drop of iodine situation (stains are used to highlight objects in a cell by adding colour
6. Place a cover slip on top (try not to get air bubbles, they’ll obstruct your view)

Question 6

How do you use a light microscope

Answer 6

1. Clip the slide onto the stage
2. Select the low/high powered objective lens (magnification)
3. Use the coarse adjustment knob to move the stage up below the objective lens
4. Look at the eyepiece. Use coarse adjustment knob to move the stage downwards until image is roughly in focus
5. Adjust the focus with the fine adjustment knob, until you get a clear image

Question 7

How to draw observations

Answer 7

Use a sharp pencil
Takes up at least half of space, drawn with clear solid lines
No colouring or shading
Subcellular structures should be drawn in proportion
Write a title of what you’re observing and magnification
Label with straight lines