Microscopy & scale (lab 1)

Question 1

What are the 2 different microscope we looked at?

Answer 1

• Leica 200 Stereomicroscope

- Nikon YS100 compound microscope

Question 2

What is the Nikon Microscope?

Answer 2

• More complex= has more moving pieces
• Gives you ability to look at something with more magnification power
• Has 4x, 10x, 40x objective (40,100,400 total magnification)

Question 3

With respect to microscopy, what are Magnification, Resolution, Contrast?

Answer 3

• Magnification= Zooming in
• Resolution= Clearer picture
• Contrast= Differentiating object from one another

Question 4

What are the functions of the Eye Pieces in a Compound Microscope?

Answer 4

• Right eye piece has pointer= helps in location of objects
• Left eye piece has focusing Diopter ring= allows to precisely adjust sharpness of image
• Eye piece magnification is 10x

Question 5

How do you calculate TOTAL MAGNIFICATION?

Answer 5

-Multiply the eye piece magnification (10) to the objective lens magnification

Question 6

Where are the Stage Controls located on the Compound Microscope?

Answer 6

• Under the right hand of the stage
• Lower knob moves it left & right
• Upper knob moves it towards you or away

Question 7

What are the Focusing Knobs on the Compound Microscope?

Answer 7

• The coarse focus knob= general up & down movement of stage, use only w/ 4x & 10x objectives
• Fine focus knob= For higher magnification objectives= moves stage vertically a very small distance

Question 8

Where is the Condenser located on the Compound Microscope?

Answer 8

-Located under the stage across from light source

Question 9

What is the Condenser on the Compound Microscope?

Answer 9

• Contains Iris Diaphragm= can be opened to permit more light to illuminate the slide
• Diaphragm is opened & closed by a lever located in the front of condenser

Question 10

What is the Rotary Switch?

Answer 10

• Located on the side of the base

- Also controls brightness

Question 11

What are the different Logarithmic scales of size described in the lab picture?

Answer 11

• X-ray crystallography
• Carbon Atom
• Electron Microscopy
• Light Microscopy
• Bacterium
• Lymphocyte
• Human egg
• Eye limit

Question 12

What is the Resolution limit for X-ray crystallography?

Answer 12

-.1 nm

Question 13

How big is a carbon Atom?

Answer 13

-.3 nm

Question 14

What is the Resolution Limit for Electron Microscopy?

Answer 14

-2nm

Question 15

What is the Resolution Limit for Light Microscopy?

Answer 15

-.5 micro m

Question 16

How big is a Bacterium?

Answer 16

-1 micro m

Question 17

How big is a Lymphocite?

Answer 17

-10 micro m

Question 18

How big is a Human Egg?

Answer 18

-100 micro m

Question 19

What is the Resolution Limit for the Human eye?

Answer 19

-500 micro m

Question 20

What is 1 Angstrom?

Answer 20

-10^-10 m