mini gene expression

Question 1

what is gene expression

Answer 1

Gene expression is the process by which the information
encoded in a gene is turned into a function. This mostly
occurs via the transcription of RNA molecules that code
for proteins or non-coding RNA molecules that serve
other functions.
Gene Expression

Question 2

Which genes are differentially expressed between healthy
and diseased tissue?

Question 3

Which genes are expressed in which tissues?

Question 4

How does gene expression change after administration of
a drug or other treatment

Question 5

what are ways to measure mrna expression

Answer 5

Ways to measure
 Quantitative rtPCR
 Microarrays
 RNA-Sequencing

Question 6

what is a good primer

Answer 6

Lack of secondary priming sites
 A melting temperature (Tm)
 Between 52°C to 65°C
 Absence of dimerization capability
 Absence of significant hairpin formation
 >3 bp
 Low specific binding at the 3’ end
 Lower GC content to avoid mispriming