Mircroscope

Question 1

What types of mircoscopes are there?

Answer 1

• Binocular or Monocular
• External or internal light sources
  -Fixed or mobile stage

Question 2

What are the different types of adjustment systems ?

Answer 2

-Fixed or mobile stage
-concentric or seperatefoucusing dials
- screw or dial condenser control

Question 3

What are the parts of a mircoscope?

Answer 3

-eye piece/ ocular lens
- Foucsing ring/ diapter adjustment
- Arm
-coarse focus
- Fine focus
-on/ off switch
- Rehostat
- Base
-Light source
-iris diaphragm
- condenser
- stage
- Slider holder/clip
-objective lens
- Nose piece

Question 4

What is apart of the support system?

Answer 4

-base and neck/arm - Provides the basic structure support
- nose piece- housing the objective len
- observation tube- supports the eye piece

Question 5

How is image produced?

Answer 5

Light from the light source first passes through the iris diaphragm and the condenser.
It then hits the specimen on the slide.
From there, the light is magnified by the objective lens and then the eyepiece,
before finally reaching the viewer’s eye.

Question 6

what does the iris diapragm (sub-stage) control?

Answer 6

controls the amount of light enterig the condenser. Help to control glare.

Question 7

What does the condensor (sub-stage) do?

Answer 7

Focuses the light onto the slide and provides good resolutions

two types
- screw type
- seperate dial

Question 8

what doese the stage do?

Answer 8

the stage holds the slide with a spring loaded clip.

Question 9

Objective lenses location

Answer 9

Objective lenses are contained within the nosepiece of the microscope.

Question 10

Number of objective lenses

Answer 10

Most microscopes have 3 or 4 objective lenses with different magnifications.

Question 11

Common objective lens magnifications

Answer 11

Common magnifications are ×4, ×10, ×40, and ×100.

Question 12

Order of magnification

Answer 12

Always start with the smallest magnification and move to the largest.

Question 13

Eyepiece lens function

Answer 13

Further magnification occurs in the eyepiece lens.

Question 14

Eyepiece magnifcation

Answer 14

The eyepiece magnification is engraved on the side and it has its own focusing ring.

Question 15

Total magnification formula

Answer 15

Total magnification = Objective lens magnification × Eyepiece magnification

Question 16

Purpose of the vernier scale on a microscope

Answer 16

It allows precise measurement and location of specimens on a slide.

Question 17

How to read the vernier scale on a microscope

Answer 17

Read the main scale just before the zero on the vernier scale.

Find the vernier line that aligns exactly with a main scale line.

Add the two values together.

Question 18

What does the main scale show?

Answer 18

The whole measurement units (usually millimetres).

Question 19

What is oil immersion?

Answer 19

Oil immersion is a technique using immersion oil with the ×100 objective lens to increase resolution.

Question 20

Why is immersion oil used?

Answer 20

It reduces light refraction, allowing more light to enter the objective lens and improving image clarity.