Mixing & Diluting

Question 1

Type of extracts

Answer 1

1. aqueous
2. freeze-dried
3. alum-precipated (allows for stronger reaction)
4. modified - not used in U.S

Question 2

Diluents for allergen extracts

Answer 2

HSA (Human Serum Albumin)

• aqueous
• freeze dried

Phenol Saline
-alum precipated

Question 3

Factors for stability for extracts

Answer 3

1. temp
2. dilution
3. diluent
4. vial volume
5. proteolytic enzymes

Question 4

Units of Potency

Answer 4

V/V
W/V
PNU
Allergy Unit (AU)
Bioequivalent Allergy Unit (BAU)

Question 5

Ideal place for mixing and diluting allergen extracts

Answer 5

Flow hoods

• horizontal
• vertical

Question 6

Source meterials for mixing and diluting

Answer 6

pollens
molds
insects
foods
mites

Question 7

Minimun requirements for label

Answer 7

1. Patient Name
2. Concentration
3. Contents
4. Numbering/ Coloring Code
5. Expiration Dates

Question 8

Label should include:

Answer 8

1. Px #
2. Vial Color
3. Vial number
4. Patient Name
5. Content of vial
6. Concentration Strength
7. Expiration

Question 9

When mixing full strength vial, what technique used

Answer 9

Sterile

Question 10

reasons for making dilutions

Answer 10

1. dropped vial
2. vial expired
3. venom allergy skin test
4. delayed testing using source antigens

Question 11

Vial volumes for dilutions

Answer 11

10mL
5mL
2mL

Question 12

Consecutive color coding for extract vials

Answer 12

Red 1:1   #1
Yellow 1:10    #2    
Blue 1:100    #3
Green 1:1000    #4
Silver 1:10,000     #5

Question 13

The expiration date of diluted extract CANNOT what?

Answer 13

exceed expiration date of source vial or diluent vial

Question 14

Expiration dates for diluations from date mixed

Answer 14

Red/Yellow -12 months

Blue/Green/Silver - 6 months

Question 15

Why are freeze dried sent

Answer 15

1. technicians are trained to dilute
2. must be refrigerated after reconstitution
3. short expiration dates

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