Modern Techniques

Question 1

A collection of cloning vectors with DNA inserts that cover some part of a genome

Answer 1

Genomic Library

Question 2

A cDNA library of expressed genes

Answer 2

Expression library

• Requires primer to match poly-A tail
• Digest all remaining RNA
• Use DNA Polymerase I
• Use S1 nuclease to cleave the turn at the end of the 3’ end

Question 3

SNP

Answer 3

Single Nucleotide Polymorphism

• A single nucleotide change in a DNA sequence that can be compared to other people’s DNA
• Allows us to find nucleotide responsible for mutant alleles, such as with sickle cell anemia

Question 4

FISH

Answer 4

Fluorescent in situ hybridization

-Biotinylated linkers are used in a DNA probe, which allows us to visualize copies of a gene in a cell or on a karyotype

Question 5

Paint Probes

Answer 5

Use biotinylated linkers with multiple DNA probes for an entire chromosome to look for translocations

Question 6

EST

Answer 6

Expressed Sequence Tag