Module 1 Karyotyping steps

Question 1

1) ___ mL ___ blood

Answer 1

5

venous

Question 2

2) Add ___ and ___

Answer 2

Phytohemagglutinin

Culture medium

Question 3

3) Culture at ___ for ___ days

Answer 3

37 C

3 days

Question 4

4) Add ___ and ___

Answer 4

Colchicine

Hypotonic saline

Question 5

5)

Answer 5

Gels fixed

Question 6

6)

Answer 6

Spread cells onto slide by dropping

Question 7

7) Digest with ___ and stain with ___

Answer 7

Trypsin

Giemsa

Question 8

8) Analyze ___ spread

Answer 8

metaphase

Question 9

9)

Answer 9

Karyotype