Microbiology-Jenn Edition > Module 8 > Flashcards

Module 8 Flashcards

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1
Q
  1. What Regulatory Protein could be present in a Positive and Negative control?
    (A) Repressor
    (B) Inducer
    (C) Activator
    (D) Corepressor
    (E) None of the above
A

b

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2
Q
  1. What is one of the secondary structures found in the attenuation step for RNA-based regulation?
    (A) Regions 1+4 Bind
    (B) Regions 2+1 Bind
    (C) Regions 1+3 Bind
    (D) Region 3+4 Bind
    (E) None of the above
A

d

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3
Q
  1. What is one of the secondary structures found in the riboswitches step for RNA-based regulation?
    (A) Regions 1+4 Bind
    (B) Regions 1+2 Bind
    (C) Regions 1+3 Bind
    (D) Region 3+4 Bind
    (E) None of the above
A

b

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4
Q
  1. Which of the following increases/decreases the amount of protein?
    (A) Post- Translational regulation
    (B) Gene regulation
    (C) Enzyme repression
    (D) Enzyme induction
    (E) None of the above
A

B

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5
Q
  1. Which of the following increases/decreases the activity of the protein?
    (A) Post-translational regulation
    (B) Gene regulation
    (C) Enzyme repression
    (D) Enzyme induction
    (E) None of the above
A

A

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6
Q
  1. Which of the following is an example of a reporter gene?
    (A) Autoflourescent protein
    (B) Cyclic AMP Receptor Protein
    (C) Arginine
    (D) Nitrogen
    (E) Green fluorescent protein
A

e

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7
Q
  1. Negative control prevents transcription when bound to DNA through___.
    (A) Binding to RNA polymerase
    (B) Guiding RNA polymerase
    (C) Blocking RNA polymerase
    (D) Degrading RNA polymerase
    (E) None of the above
A

c

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8
Q
  1. Cyclic AMP Receptor Protein is an example of___.
    (A) An activator protein
    (B) A reporter gene
    (C) A repressor gene
    (D) A signal molecule
    (E) None of the above
A

a

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9
Q
  1. Premature termination of transcription occurs___.
    (A) Before initation of transcription
    (B) After initiation of transcription and after it’s completion
    (C) After initation of transcription and before it’s completion
    (D) After translation occurs
    (E) None of the above
A

c

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10
Q
  1. ___ amounts of Trp are in form 1 of tryptophan synthesis and translation is __.
    (A) High, slow
    (B) High, Fast
    (C) Low, slow
    (D) Low, fast
    (E) None of the above
A

B

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11
Q
  1. A repressor can block transcription in the presence of…..
    (A) Corepressor
    (B) Inducer
    (C) Signal Molecules
    (D) A and B
    (E) A and C
    (F) None of the above
A

D

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12
Q
  1. For riboswitches’ secondary structure, in form 1, ______ is unbound:
    (A) Stem-Loop
    (B) Shine-Dalgarno
    (C) Ribosome Binding Site
    (D) A and B
    (E) None of the above
A

D

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13
Q
  1. If you had some synthesized mRNA and it wanted to get rid of it post-transcription but wanted to stop from being translated, what could you do?
    (A) Get a complementary antisense RNA strand
    (B) Put a repressor into the mix
    (C) Remove ribosomes
    (D) Methylate more Histones
    (E) None of the above
A

A

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14
Q
  1. Lactose binds to the repressor allowing transcription of the enzyme that can break it down. This is an example of
    (A) Negative control
    (B) Positive control
    (C) Repression control
    (D) Involving Control
    (E) None of the above
A

A

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15
Q
  1. Which internal signal molecule diffuses freely across the cytoplasmic membrane?
    (A) Autoinducers
    (B) Quorum Sensing
    (C) Water
    (D) Glucose
    (E) None of the above
A

A

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16
Q
  1. What is the function of riboswitches?
    (A) Attenuation
    (B) Porin regulation
    (C) Prevention of translation
    (D) Codon regulation
    (E) None of the above
17
Q
  1. What is the difference between gene regulation and post-translational regulation in reference to the proteins they affect?
    (A) They are the exact same thing
    (B) Gene regulation changes the amount of proteins while post-translational regulation changes the activity/function of proteins
    (C) Gene regulation changes the activity/function of proteins while post-translational regulation changes the amount of proteins
    (D) ONLY gene regulation affects proteins
    (E) ONLY post-translational regulation affects proteins
18
Q
  1. Which of the following is a factor of positive control of transcription?
    (A) Enzyme induction using catabolic enzymes
    (B) Binding and activation of a repressor by a corepressor protein
    (C) Binding of CAP to RNA polymerase
    (D) Binding of repressor protein to lac operator
    (E) None of the above
19
Q
  1. What is the difference between negative and positive control regarding the location of the respective binding site compared to the promoter?
    (A) In negative control, the site is located after the promoter; in positive control, the site is located before the promoter.
    (B) In negative control, the site is located before the promoter; in positive control, the site is located after the promoter.
    (C) Both the negative control site and positive control site are located before the promoter.
    (D) Both the negative control site and positive control site are located after the promoter.
    (E) None of the Above
20
Q
  1. Why is it important that homodimeric proteins bind to the Major Groove of DNA in both positive and negative control?
    (A) This is the only place on a DNA strand where they can bind.
    (B) It allows for high specificity in DNA binding.
    (C) To block RNA polymerase, and prevent transcription
    (D) It’s not important.
    (E) None of the above.
21
Q

Which of the following occurs when there is an excess amount of lactose?
(A) Lactose (inducer) binds to the repressor to block transcription
(B) Lactose (inducer) binds to the repressor to allow transcription
(C) The repressor is unbound and starts transcription
(D) Inducer binds to activator protein to start transcription
(E) Inducer remains unbound to the activator protein to block transcription

Microbiology-Jenn Edition
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