molecular techniques Flashcards by Sarah Heath

Q

PCR

A

Polymerase Chain Reaction. Amplify DNA via cycle after cycle of replication.

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Q

PCR steps

A

Denaturation by heat ( >90C), add a bunch of primers and Anneal, elongation (Taq polymerase, 70C), Repeat

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Q

What is an endonuclease?

A

Restriction Enzyme. sticky ends

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