Muscle Cell Differentiation

Question 1

Cell Order in Differentiation

Answer 1

Satellite cells
Myoblasts
Myotubes
Myofibers

Question 2

MyoD

Answer 2

Single gene, cause fibroblast to form muscle cells.
In muscle progenitor and muscle cells. 
Activated by Pax3.
Activates myogenin.
Auto regulatory.

Question 3

Myf5

Answer 3

Compensates for MyoD.
In myoblasts.
Autoregulatory.
Asymmetrical division of satellite cells. 
Expressed by progenitor cells.

Question 4

pax3

Answer 4

Activates MyoD.

Question 5

mrf4

Answer 5

Determination.

Acts on myf5 and myoD

Question 6

Myogenin

Answer 6

Maturation.

Actiavted by myoD and myf5

Question 7

Satellite Cells

Answer 7

Skeletal muscle stem cells.

Express pax7.

Question 8

Wnt7a

Answer 8

Drives asymmetrical (apical basal) division

Question 9

Pax7

Answer 9

Expressed by satellite cells

Question 10

Quiescence Regulation

Answer 10

Sacolemma electrical activity (silence with botulinum, more activity)

Question 11

Activation/Proliferation Regulation

Answer 11

Tension, growth, trauma change response to growth factors

Question 12

Stem Cell Niche

Answer 12

Support self renewal, prevent differentiation.

Between myofiber and ECM (quiescent)