Nucleic Acid Extraction

Question 1

Breaking the cell and nuclear membranes.

Answer 1

Cell lysis

Question 2

Nuclei acid extraction in DNA

Answer 2

DNA inorganic - salts
Organic - phenol, chloroform
Solid phase - magnetic beads, spin columns

Question 3

Nuclei acid extraction in RNA

Answer 3

Inorganic - guanidine isothiocyanate
Organic - phenol, chloroform
Solid phase - magnetic beads, spin columns

Question 4

First isolated DNA from human cells

Answer 4

Friedrich miescher

Question 5

Demonstrated the semi-conservative replication of DNA using density gradient centrifugation

Answer 5

Matthew meselson and Franklin William stahl

Question 6

Is a common approach to isolate and purify cell structures for biochemical experiments

Answer 6

Density Gradient ultracentrifugation