1
Q
Purine
A
2 rings, Adenine and Guanine
2
Q
Pyrimidines
A
1 ring, Cytosine, Thymine and Uracil
3
Q
Which carbon of deoxyribose is the deoxy
A
2
4
Q
dNTP
A
d refers to sugar nature, N refers to the base and T refers to the number of phosphates
5
Q
Chargaff’s rule
A
A pairs with T, G pairs with C
6
Q
How are nucleotides connected
A
The 3’ of one sugar binds to the phosphate on the 5’ of the next leaving the chain with a free 3’ end and an end with a phosphate attached to a 5’
7
Q
Tm
A
Melting temperature, the temperature at which half of dsDNA have denatured
BIO282
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