Nucleosomes

Question 1

how big is human DNA?

Answer 1

human DNA is less than or equal to 1 meter

Question 2

how big is the nucleus?

Answer 2

5-10 mm

Question 3

How is DNA folded?

Answer 3

DNA folded into a package almost a million times smaller

Question 4

what are nucleosomes?

Answer 4

histone proteins with ~200 base pairs of DNA

“Bead on a string”

Question 5

Nucleosomes are proteins that are what?

Answer 5

proteins are clustered at 200 bp intervals along the DNA molecule in chromatin fibers

Question 6

what does the nucleosome structure consist of?

Answer 6

eight histone molecules (octamer)
2 each of: H2A, H2B, H3, H4
146 bp DNA making two turns
50 bp Linker DNA

Question 7

what is the bead diameter of a nucleosome?

Answer 7

bead diameter of 10 nm

Question 8

what is thought to bind to linker DNA

Answer 8

histone, H1