Use of an ascending grade of alcohol
Dehydration
To preserve the cellular structure and tissue architecture in a life-like manner.
Fixation
Removal of water
Dehydration
Cross-linking or proteins
Fixation
Removal of dehydrating agent
Clearing
Leaves tissue in a transparent manner.
Wax infiltration
Makes the tissue quite hard and provides a suitable support.
Embedding
Xylene
Clearing
Osmium tetroxide
Fixation
Chloroform
Clearing
Paraffin wax
Wax infiltration
Paraplast
Wax infiltration/Impregnation
Clove oil
Clearing
Dioxane
Dehydration
Cellosolve
Dehydration
Von Ebner’s fluid
Decaltification
B-5 solution
Fixation
10% Neutral buffered formalin
Fixation
Formalin
Fixation
Ethyl alcohol
Dehydration