placing a fixed tissue in a second fixative
POST MORDANTING/SECONDARY FIXATION
Post-chromatization
POST MORDANTING/SECONDARY FIXATION
SECONDARY FIXATIVE FOR POST-CHROMATIZATION
2.5-3% potassium dichromate
acts as a mordant for better staining effects
POST-CHROMATIZATION
REMOVAL OF EXCESS FIXATIVE
WASHING OUT
REAGENTS FOR WASHING OUT
TAP WATER REMOVES EXCESS
✓ Chromates (Helly’s, Zenker’s & Flemming’s)
✓ Formalin
✓ Osmic Acid