PCR

Question 1

What does PCR stand for?

Answer 1

Polymerase Chain Reaction

Question 2

What is needed for PCR?

Answer 2

• Taq DNA polymerase
• DNA nucleotides
• RNA primers

(Basically, everything you need for replication, but the polymerase is TAQ specifically)

Question 3

Characteristic feature of Taq polymerase:

Answer 3

Does not denature at high temperatures (50-80 degrees Celsius)

Question 4

Use of PCR:

Answer 4

Rapid production of multiple copies of DNA

Question 5

Stages of PCR:

Answer 5

1) Denaturation
2) Annealing
3) Elongation

Question 6

Stages of PCR:

Answer 6

1) Denaturation (strands separate)
2) Annealing (RNA primers attatch)
3) Elongation (Taq DNA polymerase adds DNA nucleotides)