PCR

Question 1

What are the steps of PCR?

Answer 1

Denature, annealing, transcription

Question 2

What factors contribute to the denaturing phase of PCR?

Answer 2

Denature by the SEA

S –> SALT, ionic strength
E –> Elevated temperature (95C)
A –> Alkaline pH

Question 3

What temperature does primer annealing occur?

Answer 3

50-60C

Question 4

What temperature does extension occur in PCR?

Answer 4

72C

Question 5

Each cycle replicates how much DNA?

Answer 5

Doubles the amount of DNA