PCR

Question 1

Denature __ Celsius

Answer 1

94, DNA unwinds into single strands

Question 2

Anneal __ Celsius

Answer 2

55, Anneal primers to DNA strands for new strand synthesis

Question 3

Extend __ Celsius

Answer 3

72, Extend new DNA from primers w/ DNTPS

Question 4

Components

Answer 4

DNA template
Primers
dNTPs (nucleotides)
Heat stable polymerase – Taq
Buffer sloth