Anticoagulant for lavender top
ethylenediaminetetraacetic acid (EDTA or Versene).
Action of lavender top
Chelates calcium
Anticoagulant for red top
None
Action of red top
Allows blood to clot
Anticoagulant red/gray or red/ black top
none but contains separator material or gel.
Action of red/gray or red/black top
allows blood to clot and serves as a barrier between cells and serum
Anticoagulant in orange top
Thrombin
Blue top anticoagulant
Sodium citrate
Blue top action
Binds calcium
Black top anticoagulant
Sodium oxalate
Action of black top
Also binds calcium
Gray Top anticoagulant
sodium fluoride or potassium oxalate
Gray top action
inhibits glycolytic enzyme inolase and act as anticoagulant.
Yellow top anticoagulant
citrate dextrose
Yellow top anticoagulant
citrate dextrose (ACD)
Yellow top action
Preserves RBC
Green top anticoagulant
heparin (sodiumlithium or NH4)
Green Top action
inhibits thrombin activation.
Blood is drawn in the following order:
yellow top, blue top, red top, green top, lavender top and gray top
blood collection principle
Blood enters into the barrel by the pressure exerted through pulling of the plunger. On the other hand, blood enters into the evacuated tubes by evacuation.
preferred site for puncture
median cubital vein
cephalic vein
basilic vein
Angle of Draw in Venipuncture
15 to 30-degree angle
Application of Torniquet
Applied 3-4 inches above the site
yellow gauge
26G