Methods commonly used in hospitals, clinics, laboratories to destroy or control pathogens
Physical method
Most effective, inexpensive, and widely used method of sterilization of inanimate objects and materials that can withstand high temperatures.
Heat
Preferred over dry heat because of its rapid killing action. One of the most effective methods of sterilization where the steam under pressure acts as a bactericidal agent
Moist heat
Type of Moist Heat
Steam under pressure
Types of moist heat
Boiling
Types of moist heat
Fractional Sterilization
Types of moist heat
Pasteurization
The process of removing microorganisms by applying moisture-free heat which is appropriate for moisture-sensitive substances
Dry heat
Types of dry heat
Hot Air Oven
Types of dry heat
Incineration
Types of dry heat
flaming
Type of dry heat ( radiation )
Ultraviolet light
Types of dry heat ( radiation)
Ionizing Radiation
Deprive the microorganisms of moisture. Used in food preservation like dried fish and raisins
Desiccation
Lyophilization organisms are rapidly frozen, then dehydrated in high vacuum and stored in vacuum sealed containers
Freezing
From mechanical sieving. Microorganisms are not killed but merely separated from the liquid
Filtration
Types of Physical Method