PIPETTING TECHNIQUE

Question 1

MANUAL PIPETTING: Samples per hour

Answer 1

5-10 samples per hour

Question 2

(blank) using fixed volume is fast in small applications and only requires the hand of a practiced lab tech instead extra hardware

Answer 2

Manual/Hand Pipetting

Question 3

Time consuming, results can be unreliable and repetitive actions can lead to injury

Answer 3

Manual Pipetting

Question 4

Offers a way for labs to incrementally scale up production
and increase reproducibility.

Answer 4

SEMI-AUTOMATIC PIPETTES

Question 5

Only requiring the technician to move a hand probe from
vessel to vessel.

Answer 5

SEMI-AUTOMATIC PIPETTES

Question 6

Most valuable in high-throughput applications that benefit
from completely removing human movements.

Answer 6

AUTOMATION

Question 7

Can process hundreds of samples at a time and follow
highly complex methods without deviation.

Answer 7

AUTOMATION

Question 8

Protection from hazardous/infectious samples

Answer 8

AUTOMATION

Question 9

The greatest potential hazard is when is done instead of mechanical suction. Never acceptable in the clinical laboratory.

Answer 9

MOUTH PIPETTING

Question 10

(blank) and (blank) solutions can cause problems with
the measurement and delivery of samples and solutions.

Answer 10

Bubbles, Viscous

Question 11

Complement can be inactivated by

Answer 11

56° C for 30 minutes or, after 4 hours, re-inactivated by heating for 10 minutes.

Question 12

The curvature in the top surface of a liquid.

Answer 12

Meniscus

Question 13

All readings must be made with the (blank) of the
meniscus.

Answer 13

eye at the level

Question 14

TRANSFER PIPETTES

Answer 14

Volumetric Pipette
Ostwald Folin Pipette

Question 15

GRADUATED PIPETTES

Answer 15

Serological
Mohr

Question 16

Disposable plastic pipettes used to transfer small volumes of liquids

Answer 16

TRANSFER PIPETTE

Question 17

10 mL (blank) graduated to the tip

Answer 17

serological pipette

Question 18

10 mL (blank) ot graduated to the tip

Answer 18

Mohr pipette

Question 19

“To deliver” (TD) types that have the bulb closer to the center and accurately deliver a fixed volume of aqueous solution.

Answer 19

VOLUMETRIC

Question 20

TD types that have the bulb closer to the delivery tip
because they deliver viscous fluids

Answer 20

OSTWALD FOLIN

Question 21

These pipettes deliver an accurate volume by being “blown out” using a pipetting bulb

Answer 21

OSTWALD FOLIN

Question 22

A graduated pipette is a pipette with its volume, inincrements, marked along the tube.

Answer 22

GRADUATED PIPETTE

Question 23

It is used to accurately measure and transfer a volume of liquid from one container to another.

Answer 23

GRADUATED PIPETTE

Question 24

Tapered end and graduation marks on the stem

Answer 24

GRADUATED PIPETTE

Question 25

The orifice, or tip opening, is larger in the serologic pipette than in other pipettes. The rate of fall of liquid is much too fast for great accuracy or precision.

Answer 25

SEROLOGIC PIPETTES

Question 26

Calibrated to the tip and must be "blown out" to deliver entire volume. The need to blow out is indicated by the etched rings at the top of the pipette.

Answer 26

SEROLOGIC PIPETTES

Question 27

Calibrated between marks

Answer 27

MOHR PIPETTE

Question 28

Cannot be "blown out."

Answer 28

MOHR PIPETTE

Question 29

Allow rapid repetitive measurements and delivery of predetermined volumes of reagents and specimens.

Answer 29

MICROPIPETTE

Question 30

(blank) allow repeated, accurate,reproducible delivery of specimens, reagents, and other liquids requiring measurement in small amounts.

Answer 30

Piston-operated devices

Question 31

PROPER PIPETTING TECHNIQUES

Answer 31

1. Adjust pipette to the correct volume 2. Check if you are using the correct pipette tip 3. Make sure no bubbles are produced 4. Always use the pipette in a vertical position 5. No reusing of pipette tips

Question 32

AIM OF PROPER PIPETTING TECHNIQUES

Answer 32

To maximize precision and minimize contamination

Question 33

COMMON PIPETTING ERRORS

Answer 33

Loose pipette tip Tilting the pipettor Plunger quick release (will cause air bubbles) Second stop draw