Explain twinning
Twinning is when two or more crystal domains grow together in a fixed geometric relationship, causing their diffraction patterns to overlap.
Why it matters / useful:
- Can help crystals form when single crystals won’t
- Sometimes gives better diffraction due to symmetry operations
Explain omit maps
An electron-density map made after removing a part of the model density (like a residue or ligand).
Why we use it:
To avoid model bias
To confirm if a ligand or region is really there or if it was just from the model
To validate weak or uncertain density
If the omitted feature still appears in the map → the data truly supports it and it’s not just from the model
Explain anisotropic diffraction
When a crystal diffracts differently in different directions — some directions give strong reflections, others weaker.
Why it matters:
Indicates the crystal isn’t equally ordered in all directions
Affects map quality and resolution
Needs corrections (like anisotropic B-factor correction) to improve refinement and produce accurate electron-density maps