Practical: PCR

Question 1

List different Applicaitons for PCR

Answer 1

• D N A test
• Food quality testing
• diagnosis of dieases
• gene sequencing

Question 2

Explain how to analyse and interpret PCR results

Answer 2

Often done vie Electrophoresis

• A DNA ladder is run to have a comparison stripe
• See e.g. in disease the thickness of ban at target length (together with a positive and a negative control)

Question 3

What are the pros of PCR use in clinical practive?

Answer 3

• cost effeicient
• quick in diagnosis
• safe (less risk of infection for people handeling probe)

Question 4

What are the cons of use of PCR in clinical practice?

Answer 4

• might not be specific
  
  • e.g. primers amplify sequence in several mechanisms, not just in the one you want to diagnose
• DNA sequence has to be known