PRACTICALS Flashcards

(50 cards)

1
Q

what is the use of standard deviation

A

Measure range of values around the mean

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2
Q

what do overlapping standard deviation bars mean

A

the difference between two values is not significant

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3
Q

what is the use of the students t test

A

To find the difference between two means and whether it is significant

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4
Q

how to calculate degrees of freedom for the students t test

A

2n-2

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5
Q

in the students t test, when to accept the null hypothesis

A

t value is less than the critical value
means difference is due to chance

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6
Q

in the students t test, when to reject the null hypothesis

A

t value is more than the critical value
means difference is not due to chance

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7
Q

what is the use of Spearman’s
Rank correlation

A

Used to see the strength of the correlation (association) between two sets of continuous data

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8
Q

how to calculate degrees of freedom for Spearman’s
Rank correlation

A

n-2

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9
Q

in Spearman’s Rank correlation, when to reject the null hypothesis

A

if your calculated number is the same or higher than critical value then your correlation is significant and you would reject the null hypothesis

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10
Q

in Spearman’s Rank correlation, what will the result always be between

A

1 and minus 1

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11
Q

what is the use of the Chi- Squared test

A

Used to compare observed results with theoretical expected frequencies. The higher the X2 value the greater difference.

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12
Q

how to calculate degrees of freedom for the chi squared test

A

(n‐1)

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13
Q

when to reject the null hypothesis for the Chi Squared test

A

Greater value than critical value

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14
Q

how does caffeine increase heart rate in humans

A

by​ increasing release​ of excitatory neurotransmitters

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15
Q

why are daphnia used to investigate the effect of caffeine on heart rate

A

they have a ​translucent​ body through which the heartbeat can be observed

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16
Q

when investigating the effect of caffeine on heart rate in ​Daphnia, why is a coverslip not placed over the daphnia

A

to allow daphnia to access oxygen to aerobically respire

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17
Q

what happens when DCPIP is in the presence of vitamin C

A

reduced
decolourised from blue to colourless

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18
Q

what can change the permeability of a phospholipid bilayer

A

​temperature​ and ​concentration of solvents,​ like ethanol

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19
Q

how to measure the permeability of a membrane

A

​amount of beta lain pigment leaked​ from beetroot cells into an aqueous solution using a ​colorimeter​

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20
Q

what happens to beetroot cells when the cell surface membrane has a higher permeability

A

more beta lain pigment leaks out of cells

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21
Q

how to cut beetroot into identical cylinders

22
Q

in the beetroot experiment, what does a higher absorbance indicate

A

higher pigment conc
more permeable membrane

23
Q

impact of high temp on permeability of cell surface membrane

A

permeability increases
proteins in the membrane ​denature​
heat damages the bonds​ in their ​tertiary structure​
​creates gaps​ in the membrane easier for molecules to pass through it

24
Q

impact of low temp on permeability of cell surface membrane

A

permeability decreases
phospholipids have ​little energy​
​packed closely together​ to make the membrane​ rigid​
​restricts molecules​ from crossing the membrane

25
impact of VERY low temp on permeability of cell surface membrane
​ice crystals​ can form which ​pierce the cell membrane and ​increase​ the permeability
26
impact of ethanol on permeability of cell surface membrane
increasing permeability causes membrane to rupture releasing betalain pigment
27
when investigating the effect of enzyme and substrate concentrations on the rates of reactions, why is initial rate of reaction measured
​rate of an enzyme-controlled reaction is high concentration of reactants changes rapidly ​initial rate​ is the ​only point​ during the reaction when ​concentration of reactants and products is known​
28
when investigating the effect of enzyme and substrate concentrations on the rates of reactions, when does the rate plateau
all substates occupy an active site
29
where do plant cells undergo mitosis and why
at shoot and root tips in areas called ​meristems cells in the meristems are ​totipotent​ and retain the ability to ​differentiate​.
30
what stain is used to observe the stages of mitosis in a root tip squash
toluidine blue
31
why do plants require nitrate ions
used to form DNA and amino acids
32
why do plants require calcium ions
form calcium pectate for the middle lamella and in membrane permeability
33
why do plants require magnesium ions
production of chlorophyll
34
symptoms of a plant w magnesium deficiency and why
​stunted growth, yellowed leaves​ (because chlorophyll cannot be synthesised)
35
symptoms of a plant w nitrate deficiency and why
​yellowed leaves​ with ​red-brown​ cast (because chlorophyll cannot be synthesised as protein synthesis is restricted)
36
symptoms of a plant w calcium deficiency and why
​stunted growth, weakened stem​ (because the support from the cell wall is reduced and metabolism is restricted due to decreased membrane ​permeability​)
37
what is tensile strength
​maximum ​pulling force​ that can be applied to a tissue before the tissue breaks
38
when investigating antimicrobial properties of plants, why is the Petri dish lid lightly taped on, instead of around the entire dish
prevents oxygen entering​ and so promotes the growth of more harmful ​anaerobic​ bacteria
39
why is bacteria incubated at 25 celsius
incubating at 37°C (human body temperature) could enable pathogens to grow that are ​harmful to humans
40
what is the Hill reaction
in the light dependent stage of photosynthesis, electrons​ are accepted by ​NADP
41
what catalyses the Hill reaction
Dehydrogenase
42
what happens when DCPIP is present during the Hill reaction
accepts electrons rather than NADP accepting them DCPIP reduced blue to colourless
43
how to calculate Q10
dividing ​rate at T+10 degrees​ by rate at ​T degrees​
44
when investigating the effects of temperature on the development of organisms, as temp increases, what happens to number of shrimped hatched
increases​, up to an optimum of about 25 degrees Celsius. After the optimum temperature, as ​temperature increases, number of shrimp hatched will decrease​. indicates that as climate change increases temperature of sea water, the development of brine shrimps will decrease​.
45
which equipment measures the rate of respiration
respirometer
46
how does a respirometer work
adding of a drop of coloured liquid to a length of tubing as the organism respires and the volume of oxygen in the tube decreases, the ​pressure also decreases,​ and the liquid ​moves down​ the pressure gradient towards the respirometer
47
which device measures breathing and respiration
spirometer
48
how to prepare chloroplasts from leaves for Hill reaction
Take same masses of leaf Add to equal volume of isolation medium Homogenise (e.g. blend / grind in pestle and mortar) for same length of time Filter suspensions of plant material (through muslin) or centrifuge to concentrate chloroplasts Resuspend pellet of chloroplasts if centrifuged
49
what is the purpose of isolation medium in the Hill reaction
prevent cell damage due to osmosis or extreme pH
50
explain what a p value of 0.05 means
5%/ 1 in 20 probability the results occurred by chance