Which of the following techniques are used in differentiation of fungi?
- molecular and culture-based
- serologic assays
- antibody-detected
- calcofluor white staining
molecular and culture-based
standard specimen for diagnosis of Pneumocystis spp.
bronchoalveolar lavage
which is not a biochemical characteristics of C. neoformans?
- latex agglutination positive
- positive urease
- inability to grow in birdseed agar
- negative in nitrate reduction
inability to grow in birdseed agar
sexual diagnostic stage of P. boydii
cleistothecia
molecular target for fusarium
EF-1
yeast reported to cause mycotic keratitis
Candida albicans
Aspergillus spp., Mucor spp., Penicillum spp., and Rhizopus spp., are collectively known to cause?
ear infections
oncomycosis
Candida spp. that can give (+) germ tube reaction and (-) trehalose
C. dublinensis
fungal stain for melanin
Masson Fontana
beaver’s tail-like conidia
Epidermophyton flocossum
spaghetti and meatballs fungus
Malassezia furfur
Histoplasma capsulatum exoantigen
H&M
San Joaquin Valley fever
Coccidioides immitis
Gilchrist’s disease
Blastomyces dermatitidis
Germ Tube Test Procedure
- Mix fungal colony with serum
- Incubate for 3hrs
- Observe in HPO
Biosafety requirement for Histoplasma capsulatum
BSL-3
Usual cause of meningitis:
- Coccidioides
- Candida
- C. neoformans
- All of the above
All of them
fungi in UTI
Candida spp.
which group of molds can be ruled out when septate hyphae is seen?
- dematiacious
- zygomycetes
- dermatophytes
- dimorphic molds
zygomycetes
Best for initial identification of aspergillosis by soluble antigen testing
serum
- growth on SBA: dimorphic
- mold: thick, spherical tuberculated macroconidia
Histoplasma capsulatum
